| Added by | llasorsa |
|---|---|
| Group name | EquipeMY |
| Item Type | Journal Article |
| Title | Monitoring levels of circulating cell-free DNA in patients with metastatic colorectal cancer as a potential biomarker of responses to regorafenib treatment |
| Creator | Pastor et al. |
| Author | Brice Pastor |
| Author | Thierry André |
| Author | Julie Henriques |
| Author | Isabelle Trouilloud |
| Author | Christophe Tournigand |
| Author | Marine Jary |
| Author | Thibault Mazard |
| Author | Christophe Louvet |
| Author | Simon Azan |
| Author | Audrey Bauer |
| Author | Cynthia Sanchez |
| Author | Dewi Vernerey |
| Author | Alain R. Thierry |
| Author | Antoine Adenis |
| Abstract | Circulating cell-free DNA (cfDNA) contains circulating tumor DNA (ctDNA), which can be obtained from serial liquid biopsies to enable tumor genome analysis throughout the course of treatment. We investigated cfDNA and mutant ctDNA as potential biomarkers to predict the best outcomes of regorafenib-treated metastatic colorectal cancer (mCRC) patients. We analyzed longitudinally collected plasma cfDNA of 43 mCRC patients prospectively enrolled in the phase II TEXCAN trial by IntPlex qPCR. Qualitative (KRAS, NRAS, BRAFV600E mutations) and quantitative (total cfDNA concentration, mutant ctDNA concentration, mutant ctDNA fraction) parameters were correlated with overall survival (OS) and progression-free survival (PFS). When examined as classes or continuous variables, the concentrations of total cfDNA, mutant ctDNA, and, partly, mutant ctDNA fraction prior to regorafenib treatment correlated with OS. Patients with baseline cfDNA > 26 ngˇmL-1 had shorter OS than those with cfDNA value below this threshold (4.0 vs 6.9 months; log-rank P = 0.0366). Patients with baseline mutant ctDNA > 2 ngˇmL-1 had shorter OS than those with mutant ctDNA below this threshold (log-rank P = 0.0154). We show that pretreatment cfDNA and mutant ctDNA levels may identify mCRC patients that may benefit from regorafenib treatment. |
| Publication | Molecular Oncology |
| Volume | 15 |
| Issue | 9 |
| Pages | 2401-2411 |
| Date | 2021-09 |
| Journal Abbr | Mol Oncol |
| Language | eng |
| DOI | 10.1002/1878-0261.12972 |
| ISSN | 1878-0261 |
| Library Catalog | PubMed |
| Extra | PMID: 33934494 PMCID: PMC8410523 |
| Tags | Aged, Antineoplastic Agents, Biomarkers, Tumor, Cell-Free Nucleic Acids, colorectal cancer, Colorectal Neoplasms, Female, Humans, Male, Middle Aged, Neoplasm Metastasis, Phenylurea Compounds, Pyridines, Survival Analysis |
| Date Added | 2022/09/29 - 15:23:23 |
| Date Modified | 2024/10/10 - 16:52:32 |
| Notes and Attachments | PubMed entry (Attachment) Texte intégral (Attachment) Texte intégral (Attachment) |
Institut de Recherche en Cancérologie de
