| Added by | Cavailles |
|---|---|
| Group name | EquipeVC |
| Item Type | Journal Article |
| Title | Development of new biocompatible 3D printed graphene oxide-based scaffolds |
| Creator | Belaid et al. |
| Author | Habib Belaid |
| Author | Sakthivel Nagarajan |
| Author | Catherine Teyssier |
| Author | Carole Barou |
| Author | Hélène Garay |
| Author | Vincent Huon |
| Author | David Cornu |
| Author | Vincent Cavaillès |
| Author | Mikhael Bechelany |
| Abstract | The aim of this work was to develop a bioresorbable, biodegradable and biocompatible synthetic polymer with good mechanical properties for bone tissue engineering applications. Polylactic acid (PLA) scaffolds were generated by 3D printing using the fused deposition modelling method, and reinforced by incorporation of graphene oxide (GO). Morphological analysis by scanning electron microscopy indicated that the scaffold average pore size was between 400 and 500 ?m. Topography imaging revealed a rougher surface upon GO incorporation (Sa = 5.8 ?m for PLA scaffolds, and of 9.9 ?m for PLA scaffolds with 0.2% GO), and contact angle measurements showed a transition from a hydrophobic surface (pure PLA scaffolds) to a hydrophilic surface after GO incorporation. PLA thermomechanical properties were enhanced by GO incorporation, as shown by the 70 °C increase of the degradation peak (thermal gravimetric analysis). However, GO incorporation did not change significantly the melting point assessed by differential scanning calorimetry. Physicochemical analyses by X-ray diffraction and Raman spectroscopy confirmed the filler presence. Tensile testing demonstrated that the mechanical properties were improved upon GO incorporation (30% increase of the Young's modulus with 0.3% GO). Cell viability, attachment, proliferation and differentiation assays using MG-63 osteosarcoma cells showed that PLA/GO scaffolds were biocompatible and that they promoted cell proliferation and mineralization more efficiently than pure PLA scaffolds. In conclusion, this new 3D printed nanocomposite is a promising scaffold with adequate mechanical properties and cytocompatibility which may allow bone formation. |
| Publication | Materials Science & Engineering. C, Materials for Biological Applications |
| Volume | 110 |
| Pages | 110595 |
| Date | 2020-05 |
| Journal Abbr | Mater Sci Eng C Mater Biol Appl |
| Language | eng |
| DOI | 10.1016/j.msec.2019.110595 |
| ISSN | 1873-0191 |
| Library Catalog | PubMed |
| Extra | PMID: 32204059 |
| Tags | 3D printing, Animals, Biocompatibility, Bone and Bones, Cell Line, Tumor, Graphene oxide, Graphite, Humans, Mice, Nanocomposite, original, Osteoblasts, own, Polylactic acid, Printing, Three-Dimensional, Tissue Engineering, Tissue Scaffolds, top |
| Date Added | 2021/09/01 - 17:16:38 |
| Date Modified | 2023/11/21 - 13:58:36 |
| Notes and Attachments | PubMed entry (Attachment) Texte intégral (Attachment) |
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