| Added by | mollevi |
|---|---|
| Group name | PlateformeIPAM |
| Item Type | Journal Article |
| Title | The p53 isoform ?133p53? promotes cancer stem cell potential |
| Creator | Arsic et al. |
| Author | Nikola Arsic |
| Author | Gilles Gadea |
| Author | E. Louise Lagerqvist |
| Author | Muriel Busson |
| Author | Nathalie Cahuzac |
| Author | Carsten Brock |
| Author | Veronique Gire |
| Author | Julie Pannequin |
| Author | Pierre Roux |
| Abstract | Cancer stem cells (CSC) are responsible for cancer chemoresistance and metastasis formation. Here we report that ?133p53?, a TP53 splice variant, enhanced cancer cell stemness in MCF-7 breast cancer cells, while its depletion reduced it. ?133p53? stimulated the expression of the key pluripotency factors SOX2, OCT3/4, and NANOG. Similarly, in highly metastatic breast cancer cells, aggressiveness was coupled with enhanced CSC potential and ?133p53? expression. Like in MCF-7 cells, SOX2, OCT3/4, and NANOG expression were positively regulated by ?133p53? in these cells. Finally, treatment of MCF-7 cells with etoposide, a cytotoxic anti-cancer drug, increased CSC formation and SOX2, OCT3/4, and NANOG expression via ?133p53, thus potentially increasing the risk of cancer recurrence. Our findings show that ?133p53? supports CSC potential. Moreover, they indicate that the TP53 gene, which is considered a major tumor suppressor gene, also acts as an oncogene via the ?133p53? isoform. |
| Publication | Stem Cell Reports |
| Volume | 4 |
| Issue | 4 |
| Pages | 531-540 |
| Date | Apr 14, 2015 |
| Journal Abbr | Stem Cell Reports |
| Language | eng |
| DOI | 10.1016/j.stemcr.2015.02.001 |
| ISSN | 2213-6711 |
| Library Catalog | PubMed |
| Extra | PMID: 25754205 PMCID: PMC4400643 |
| Tags | Alternative Splicing, Antineoplastic Agents, Breast Neoplasms, Cell Line, Tumor, Cell Movement, Cell Self Renewal, Female, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Humans, MCF-7 Cells, Neoplastic Stem Cells, original, RNA Isoforms, Spheroids, Cellular, Transcription Factors, Tumor Cells, Cultured, Tumor Suppressor Protein p53 |
| Date Added | 2019/06/04 - 17:01:34 |
| Date Modified | 2019/06/05 - 11:03:38 |
Institut de Recherche en Cancérologie de
