| Added by | jacques.colinge |
|---|---|
| Group name | EquipeJC |
| Item Type | Journal Article |
| Title | Detection of soluble biomarkers of pancreatic cancer in endoscopic ultrasound-guided fine-needle aspiration samples |
| Creator | Souche et al. |
| Author | Guillaume Tosato |
| Author | Benjamin Rivière |
| Author | Jean-Christophe Valats |
| Author | Antoine Debourdeau |
| Author | Nicolas Flori |
| Author | Didier Pourquier |
| Author | Jean-Michel Fabre |
| Author | Eric Assenat |
| Author | Jacques Colinge |
| Author | Andrei Turtoi |
| Abstract | BACKGROUND: Biomarkers are urgently needed for pancreatic ductal adenocarcinoma (PDAC). Endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) is the cornerstone for diagnosing PDAC. We developed a method for discovery of PDAC biomarkers using the discarded EUS-FNA liquid. METHODS: This retrospective study included 58 patients with suspected pancreatic lesions who underwent EUS-FNA. Protein extracts from EUS-FNA liquid were analyzed by mass spectrometry. Proteomic and clinical data were modeled by supervised statistical learning to identify protein markers and clinical variables that distinguish PDAC. RESULTS: Statistical modeling revealed a protein signature for PDAC screening that achieved high sensitivity and specificity (0.92, 95?% confidence interval [CI] 0.79-0.98, and 0.85, 95?%CI 0.67-0.93, respectively). We also developed a protein signature score (PSS) to guide PDAC diagnosis. In combination with patient age, the PSS achieved 100?% certainty in correctly identifying PDAC patients?>?54 years. In addition, 3?/4 inconclusive EUS-FNA biopsies were correctly identified using PSS. CONCLUSIONS: EUS-FNA-derived fluid is a rich source of PDAC proteins with biomarker potential. The PSS requires further validation and verification of the feasibility of measuring these proteins in patient sera. |
| Publication | Endoscopy |
| Volume | 54 |
| Issue | 5 |
| Pages | 503-508 |
| Date | 2022-05 |
| Journal Abbr | Endoscopy |
| Language | eng |
| DOI | 10.1055/a-1550-2503 |
| ISSN | 1438-8812 |
| Library Catalog | PubMed |
| Extra | PMID: 34448184 |
| Tags | Biomarkers, Carcinoma, Pancreatic Ductal, corresponding, Endoscopic Ultrasound-Guided Fine Needle Aspiration, Humans, Middle Aged, original, Pancreatic Neoplasms, phd, Retrospective Studies |
| Date Added | 2022/07/29 - 09:14:08 |
| Date Modified | 2024/09/08 - 17:08:21 |
| Notes and Attachments | PubMed entry (Attachment) |
Institut de Recherche en Cancérologie de
