| Added by | pcoopman |
|---|---|
| Group name | EquipePC |
| Item Type | Journal Article |
| Title | The kinesin KIF1C transports APC-dependent mRNAs to cell protrusions |
| Creator | Pichon et al. |
| Author | Xavier Pichon |
| Author | Konstadinos Moissoglu |
| Author | Emeline Coleno |
| Author | Tianhong Wang |
| Author | Arthur Imbert |
| Author | Marie-Cécile Robert |
| Author | Marion Peter |
| Author | Racha Chouaib |
| Author | Thomas Walter |
| Author | Florian Mueller |
| Author | Kazem Zibara |
| Author | Edouard Bertrand |
| Author | Stavroula Mili |
| Abstract | RNA localization and local translation are important for numerous cellular functions. In mammals, a class of mRNAs localize to cytoplasmic protrusions in an APC-dependent manner, with roles during cell migration. Here, we investigated this localization mechanism. We found that the KIF1C motor interacts with APC-dependent mRNAs and is required for their localization. Live cell imaging revealed rapid, active transport of single mRNAs over long distances that requires both microtubules and KIF1C. Two-color imaging directly revealed single mRNAs transported by single KIF1C motors, with the 3'UTR being sufficient to trigger KIF1C-dependent RNA transport and localization. Moreover, KIF1C remained associated with peripheral, multimeric RNA clusters and was required for their formation. These results reveal a widespread RNA transport pathway in mammalian cells, in which the KIF1C motor has a dual role in transporting RNAs and clustering them within cytoplasmic protrusions. Interestingly, KIF1C also transports its own mRNA, suggesting a possible feedback loop acting at the level of mRNA transport. |
| Publication | RNA (New York, N.Y.) |
| Volume | 27 |
| Issue | 12 |
| Pages | 1528-1544 |
| Date | 2021-12 |
| Journal Abbr | RNA |
| Language | eng |
| DOI | 10.1261/rna.078576.120 |
| ISSN | 1469-9001 |
| Library Catalog | PubMed |
| Extra | PMID: 34493599 PMCID: PMC8594469 |
| Tags | Adenomatous Polyposis Coli Protein, Animals, Cell Surface Extensions, Cytoplasm, cytoplasmic protrusions, HeLa Cells, Humans, Kinesins, local translation, original, RNA localization, RNA transport, RNA, Messenger |
| Date Added | 2023/11/15 - 18:24:19 |
| Date Modified | 2023/11/15 - 18:25:06 |
| Notes and Attachments | PubMed entry (Attachment) Texte intégral (Attachment) |
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