| Added by | pcoopman |
|---|---|
| Group name | EquipePC |
| Item Type | Journal Article |
| Title | A Dual Protein-mRNA Localization Screen Reveals Compartmentalized Translation and Widespread Co-translational RNA Targeting |
| Creator | Chouaib et al. |
| Author | Racha Chouaib |
| Author | Adham Safieddine |
| Author | Xavier Pichon |
| Author | Arthur Imbert |
| Author | Oh Sung Kwon |
| Author | Aubin Samacoits |
| Author | Abdel-Meneem Traboulsi |
| Author | Marie-Cécile Robert |
| Author | Nikolay Tsanov |
| Author | Emeline Coleno |
| Author | Ina Poser |
| Author | Christophe Zimmer |
| Author | Anthony Hyman |
| Author | Hervé Le Hir |
| Author | Kazem Zibara |
| Author | Marion Peter |
| Author | Florian Mueller |
| Author | Thomas Walter |
| Author | Edouard Bertrand |
| Abstract | Local translation allows spatial control of gene expression. Here, we performed a dual protein-mRNA localization screen, using smFISH on 523 human cell lines expressing GFP-tagged genes. 32 mRNAs displayed specific cytoplasmic localizations with local translation at unexpected locations, including cytoplasmic protrusions, cell edges, endosomes, Golgi, the nuclear envelope, and centrosomes, the latter being cell-cycle-dependent. Automated classification of mRNA localization patterns revealed a high degree of intercellular heterogeneity. Surprisingly, mRNA localization frequently required ongoing translation, indicating widespread co-translational RNA targeting. Interestingly, while P-body accumulation was frequent (15 mRNAs), four mRNAs accumulated in foci that were distinct structures. These foci lacked the mature protein, but nascent polypeptide imaging showed that they were specialized translation factories. For ?-catenin, foci formation was regulated by Wnt, relied on APC-dependent polysome aggregation, and led to nascent protein degradation. Thus, translation factories uniquely regulate nascent protein metabolism and create a fine granular compartmentalization of translation. |
| Publication | Developmental Cell |
| Volume | 54 |
| Issue | 6 |
| Pages | 773-791.e5 |
| Date | 2020-09-28 |
| Journal Abbr | Dev Cell |
| Language | eng |
| DOI | 10.1016/j.devcel.2020.07.010 |
| ISSN | 1878-1551 |
| Library Catalog | PubMed |
| Extra | PMID: 32783880 |
| Tags | ASPM, Beta-catenin, Cell Line, Centrosome, co-translational targeting, Gene Expression Regulation, Humans, local translation, original, Polyribosomes, Protein Biosynthesis, Protein Transport, RNA, RNA localization, RNA transport, RNA, Messenger, smFISH, translation factories |
| Date Added | 2023/11/15 - 18:34:42 |
| Date Modified | 2023/11/15 - 18:35:15 |
| Notes and Attachments | PubMed entry (Attachment) Texte intégral (Attachment) |
Institut de Recherche en Cancérologie de
