| Added by | liaudet-coopman |
|---|---|
| Group name | EquipeELC |
| Item Type | Journal Article |
| Title | CD99 isoforms regulate CD1a expression in human monocyte-derived DCs through ATF-2/CREB-1 phosphorylation |
| Creator | Mahiddine et al. |
| Author | Karim Mahiddine |
| Author | Aude Mallavialle |
| Author | Hanen Bziouech |
| Author | Frédéric Larbret |
| Author | Alain Bernard |
| Author | Ghislaine Bernard |
| Abstract | CD1a expression is considered one of the major characteristics qualifying in vitro human dendritic cells (DCs) during their generation process. Here, we report that CD1A transcription is regulated by a mechanism involving the long and short isoforms of CD99. Using a lentiviral construct encoding for a CD99 short hairpin RNA, we were able to inhibit CD99 expression in human primary DCs. In such cells, CD1a membrane expression increased and CD1A transcripts were much higher in abundance compared to cells expressing CD99 long form (CD99LF). We also show that CD1A transcription is accompanied by a switch in expression from CD99LF to expression at comparable levels of both CD99 isoforms during immature DCs generation in vitro. We demonstrate that CD99LF maintains a lower level of CD1A transcription by up-regulating the phosphorylated form of the ATF-2 transcription factor and that CD99 short form (SF) is required to counteract this regulatory mechanism. Elucidation of the molecular mechanisms related to CD99 alternative splicing will be very helpful to better understand the transcriptional regulatory mechanism of CD1a molecules during DCs differentiation and its involvement in the immune response. |
| Publication | European Journal of Immunology |
| Volume | 46 |
| Issue | 6 |
| Pages | 1460-1471 |
| Date | 06 2016 |
| Journal Abbr | Eur. J. Immunol. |
| Language | eng |
| DOI | 10.1002/eji.201546143 |
| ISSN | 1521-4141 |
| Library Catalog | PubMed |
| Extra | PMID: 27094031 |
| Tags | 12E7 Antigen, Activating Transcription Factor 2, alternative splicing-ATF2-CREB1, Antigens, CD1, CD99, Cell Differentiation, Cell Line, Cells, Cultured, Cyclic AMP Response Element-Binding Protein, Dendritic Cells, Gene Expression Regulation, Humans, Monocytes, Nonclassical MHC, original, Phosphorylation, Protein Isoforms, Transcription, Genetic |
| Date Added | 2018/09/26 - 14:32:42 |
| Date Modified | 2019/05/29 - 12:12:54 |
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