| Added by | chplanque |
|---|---|
| Group name | EquipeADV |
| Item Type | Journal Article |
| Title | The cell proliferation antigen Ki-67 organises heterochromatin |
| Creator | Sobecki et al. |
| Author | Michal Sobecki |
| Author | Karim Mrouj |
| Author | Alain Camasses |
| Author | Nikolaos Parisis |
| Author | Emilien Nicolas |
| Author | François Gerbe |
| Author | Susana Prieto |
| Author | Liliana Krasinska |
| Author | Alexandre David |
| Author | Manuel Eguren |
| Author | Marie-Christine Birling |
| Author | Serge Urbach |
| Author | Sonia Hem |
| Author | Jérôme Déjardin |
| Author | Marcos Malumbres |
| Author | Philippe Jay |
| Author | Vjekoslav Dulic |
| Author | Denis Lj Lafontaine |
| Author | Robert Feil |
| Author | Daniel Fisher |
| Abstract | Elife. 2016 Mar 7;5:e13722. doi: 10.7554/eLife.13722. ABSTRACT Antigen Ki-67 is a nuclear protein expressed in proliferating mammalian cells. It is widely used in cancer histopathology but its functions remain unclear. Here, we show that Ki-67 controls heterochromatin organisation. Altering Ki-67 expression levels did not significantly affect cell proliferation in vivo. Ki-67 mutant mice developed normally and cells lacking Ki-67 proliferated efficiently. Conversely, upregulation of Ki-67 expression in differentiated tissues did not prevent cell cycle arrest. Ki-67 interactors included proteins involved in nucleolar processes and chromatin regulators. Ki-67 depletion disrupted nucleologenesis but did not inhibit pre-rRNA processing. In contrast, it altered gene expression. Ki-67 silencing also had wide-ranging effects on chromatin organisation, disrupting heterochromatin compaction and long-range genomic interactions. Trimethylation of histone H3K9 and H4K20 was relocalised within the nucleus. Finally, overexpression of human or Xenopus Ki-67 induced ectopic heterochromatin formation. Altogether, our results suggest that Ki-67 expression in proliferating cells spatially organises heterochromatin, thereby controlling gene expression. PMID:26949251 | PMC:PMC4841783 | DOI:10.7554/eLife.13722 |
| Publication | eLife |
| Date | 2016-03-08 00:00:00 |
| Language | en |
| DOI | 10.7554/eLife.13722 |
| URL | https://pubmed.ncbi.nlm.nih.gov/26949251/?utm_source=Firefox&utm_medium=rss&utm_campaign=pubmed-2&utm_content=16WqI68KDOBEDOCNE1P3RxYQYPTwTutpbvUaDhoUuYPuJd459j&fc=20250120064306&ff=20250120064533&v=2.18.0.post9+e462414 |
| Tags | original |
| Date Added | 2025/01/20 - 14:26:36 |
| Date Modified | 2025/01/21 - 11:09:27 |
Institut de Recherche en Cancérologie de
