| Added by | pmartino |
|---|---|
| Group name | EquipePM |
| Item Type | Journal Article |
| Title | Construction of a Synthetic Antibody Gene Library for the Selection of Intrabodies and Antibodies |
| Creator | Caucheteur et al. |
| Author | Déborah Caucheteur |
| Author | Gautier Robin |
| Author | Vincent Parez |
| Author | Pierre Martineau |
| Abstract | Libraries of antibody fragments displayed on filamentous phages have proved their value to generate human antibodies against virtually any target. We describe here a simple protocol to make large and diverse libraries based on a single or a limited number of frameworks. The approach is flexible enough to be used with any antibody format, either single-chain (scFv, VHH) or multi-chain (Fv, Fab, (Fab')2), and to target in a single step the six complementarity-determining regions-or any other part-of the antibody molecule. Using this protocol, libraries larger than 1010 can be easily constructed in a single week. |
| Publication | Methods in Molecular Biology (Clifton, N.J.) |
| Volume | 1701 |
| Pages | 239-253 |
| Date | 2018 |
| Journal Abbr | Methods Mol. Biol. |
| Language | eng |
| DOI | 10.1007/978-1-4939-7447-4_12 |
| ISSN | 1940-6029 |
| Library Catalog | PubMed |
| Extra | PMID: 29116508 |
| Tags | Animals, Antibody fragment, Cloning, Molecular, corresponding, first, Gene Library, Humans, Kunkel mutagenesis, last, original, Peptide Library, Phage-display, Single-Chain Antibodies, Single-chain Fv, Synthetic library, top |
| Date Added | 2018/07/20 - 09:37:47 |
| Date Modified | 2021/09/01 - 09:39:06 |
| Notes and Attachments | Caucheteur et al. - 2018 - Construction of a Synthetic Antibody Gene Library .pdf (Attachment) |
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