| Added by | standudu |
|---|---|
| Group name | EquipeCTCS |
| Item Type | Journal Article |
| Title | PRMT5-mediated histone H4 arginine-3 symmetrical dimethylation marks chromatin at G + C-rich regions of the mouse genome |
| Creator | Girardot et al. |
| Author | Michael Girardot |
| Author | Ryutaro Hirasawa |
| Author | Salim Kacem |
| Author | Lauriane Fritsch |
| Author | Julien Pontis |
| Author | Satya K. Kota |
| Author | Doria Filipponi |
| Author | Eric Fabbrizio |
| Author | Claude Sardet |
| Author | Felix Lohmann |
| Author | Shilpa Kadam |
| Author | Slimane Ait-Si-Ali |
| Author | Robert Feil |
| Abstract | Symmetrical dimethylation on arginine-3 of histone H4 (H4R3me2s) has been reported to occur at several repressed genes, but its specific regulation and genomic distribution remained unclear. Here, we show that the type-II protein arginine methyltransferase PRMT5 controls H4R3me2s in mouse embryonic fibroblasts (MEFs). In these differentiated cells, we find that the genome-wide pattern of H4R3me2s is highly similar to that in embryonic stem cells. In both the cell types, H4R3me2s peaks are detected predominantly at G + C-rich regions. Promoters are consistently marked by H4R3me2s, independently of transcriptional activity. Remarkably, H4R3me2s is mono-allelic at imprinting control regions (ICRs), at which it marks the same parental allele as H3K9me3, H4K20me3 and DNA methylation. These repressive chromatin modifications are regulated independently, however, since PRMT5-depletion in MEFs resulted in loss of H4R3me2s, without affecting H3K9me3, H4K20me3 or DNA methylation. Conversely, depletion of ESET (KMT1E) or SUV420H1/H2 (KMT5B/C) affected H3K9me3 and H4K20me3, respectively, without altering H4R3me2s at ICRs. Combined, our data indicate that PRMT5-mediated H4R3me2s uniquely marks the mammalian genome, mostly at G + C-rich regions, and independently from transcriptional activity or chromatin repression. Furthermore, comparative bioinformatics analyses suggest a putative role of PRMT5-mediated H4R3me2s in chromatin configuration in the nucleus. |
| Publication | Nucleic Acids Research |
| Volume | 42 |
| Issue | 1 |
| Pages | 235-248 |
| Date | Jan 2014 |
| Journal Abbr | Nucleic Acids Res. |
| Language | eng |
| DOI | 10.1093/nar/gkt884 |
| ISSN | 1362-4962 |
| Library Catalog | PubMed |
| Extra | PMID: 24097435 PMCID: PMC3874197 |
| Tags | Alleles, Animals, Arginine, Cells, Cultured, Chromatin, DNA Methylation, Fibroblasts, GC Rich Sequence, Genomic Imprinting, Histones, Methylation, Mice, original, Promoter Regions, Genetic, Protein Methyltransferases, Protein-Arginine N-Methyltransferases |
| Date Added | 2019/05/31 - 11:47:28 |
| Date Modified | 2019/05/31 - 11:47:57 |
| Notes and Attachments | PubMed entry (Attachment) Texte intégral (Attachment) |
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