| Added by | pcoopman |
|---|---|
| Last modified by | standudu |
| Group name | EquipePC |
| Item Type | Journal Article |
| Title | Synovial-Fluid miRNA Signature for Diagnosis of Juvenile Idiopathic Arthritis |
| Creator | Nziza et al. |
| Author | Nadége Nziza |
| Author | Eric Jeziorski |
| Author | Marion Delpont |
| Author | Maïlys Cren |
| Author | Hugues Chevassus |
| Author | Aurélia Carbasse |
| Author | Perrine Mahe |
| Author | Hamouda Abassi |
| Author | Pauline Joly-Monrigal |
| Author | Eric Schordan |
| Author | Alain Mangé |
| Author | Christian Jorgensen |
| Author | Florence Apparailly |
| Author | Isabelle Duroux-Richard |
| Abstract | Juvenile idiopathic arthritis (JIA) is the most common chronic inflammatory rheumatism in childhood; microRNAs (miRNAs) have been proposed as diagnostic biomarkers. Although joints are the primary targets for JIA, a synovial fluid-based miRNA signature has never been studied. We aim to identify miRNA biomarkers in JIA by comparing synovial fluid and serum samples from children with JIA and K.kingae septic arthritis (SA). With next-generation high-throughput sequencing, we measured the absolute levels of 2083 miRNAs in synovial fluid and serum from an exploratory cohort of children and validated differentially expressed miRNAs in a replication study by using RT-qPCR. We identified a 19-miRNA signature only in synovial fluid samples that was significantly deregulated, with at least 2-fold change in expression, in JIA versus SA (p < 0.01). The combination of miR-6764-5p, miR-155, and miR-146a-5p expression in synovial fluid yielded an area under the receiver operating characteristic curve of 1 (95% CI 0.978 to 1), thereby perfectly differentiating JIA from SA in children. We propose, for the first time, a synovial fluid-specific miRNA signature for JIA and associated signaling pathways that may indicate potential biomarkers to assist in the classification and differential diagnosis of JIA and help in understanding JIA pathogenesis. |
| Publication | Cells |
| Volume | 8 |
| Issue | 12 |
| Date | Nov 26, 2019 |
| Journal Abbr | Cells |
| Language | eng |
| DOI | 10.3390/cells8121521 |
| ISSN | 2073-4409 |
| Library Catalog | PubMed |
| Extra | 00000 PMID: 31779271 |
| Tags | juvenile arthritis, miRNA, original, synovial fluid |
| Date Added | 2019/12/13 - 14:32:49 |
| Date Modified | 2020/10/04 - 14:01:59 |
| Notes and Attachments | PubMed entry (Attachment) Texte intégral (Attachment) |
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