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Epitranscriptomics & Cancer Adaptation : A.David

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Our research work focuses on the contribution of post-transcriptional mechanisms on cancer cell adaptation, in particular RNA epigenetic & translational control.

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Added by llasorsa
Group name EquipeMY
Item Type Journal Article
Title PTPN13 Participates in the Regulation of Epithelial-Mesenchymal Transition and Platinum Sensitivity in High-Grade Serous Ovarian Carcinoma Cells
Creator Aptecar et al.
Author Leticia Aptecar
Author Carole Puech
Author Evelyne Lopez-Crapez
Author Marion Peter
Author Peter Coopman
Author Véronique D'Hondt
Author Gilles Freiss
Abstract Epithelial ovarian cancer (EOC) is the leading cause of death from gynecological cancers in Western countries. High-Grade Serous Ovarian Carcinoma (HGSOC) accounts for 60-70% of EOC and is the most aggressive subtype. Reduced PTPN13 expression levels have been previously correlated with worse prognosis in HGSOC. However, PTPN13's exact role and mechanism of action in these tumors remained to be investigated. To elucidate PTPN13's role in HGSOC aggressiveness, we used isogenic PTPN13-overexpressing clones of the OVCAR-8 cell line, which poorly expresses PTPN13, and also PTPN13 CRISPR/Cas9-mediated knockout/knockdown clones of the KURAMOCHI cell line, which strongly expresses PTPN13. We investigated their migratory and invasive capacity using a wound healing assay, their mesenchymal-epithelial transition (EMT) status using microscopy and RT-qPCR, and their sensitivity to chemotherapeutic drugs used for HGSOC. We found that (i) PTPN13 knockout/knockdown increased migration and invasion in KURAMOCHI cells that also displayed a more mesenchymal phenotype and increased expression of the SLUG, SNAIL, ZEB-1, and ZEB-2 EMT master genes; and (ii) PTPN13 expression increased the platinum sensitivity of HGSOC cells. These results suggest that PTPN13 might be a predictive marker of response to platinum salts in HGSOC.
Publication International Journal of Molecular Sciences
Volume 24
Issue 20
Pages 15413
Date 2023-10-21
Journal Abbr Int J Mol Sci
Language eng
DOI 10.3390/ijms242015413
ISSN 1422-0067
Library Catalog PubMed
Extra PMID: 37895093 PMCID: PMC10607604
Tags Carcinoma, Ovarian Epithelial, cell aggressiveness, Cell Line, Tumor, Cystadenocarcinoma, Serous, drug sensitivity, EMT, Epithelial-Mesenchymal Transition, Female, HGSOC, Humans, Ovarian Neoplasms, Phenotype, Protein Tyrosine Phosphatase, Non-Receptor Type 13, PTPN13
Date Added 2023/11/15 - 14:52:59
Date Modified 2023/11/15 - 14:52:59
Notes and Attachments PubMed entry (Attachment)
Texte intégral (Attachment)


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