| Added by | pcoopman |
|---|---|
| Group name | EquipePC |
| Item Type | Journal Article |
| Title | Exploring the Significance of the Exon 4-Skipping Isoform of the ZNF217 Oncogene in Breast Cancer |
| Creator | Bellanger et al. |
| Author | Aurélie Bellanger |
| Author | Diep T. Le |
| Author | Julie Vendrell |
| Author | Anne Wierinckx |
| Author | L?rinc S. Pongor |
| Author | Jérôme Solassol |
| Author | Joël Lachuer |
| Author | Philippe Clezardin |
| Author | Balázs Gy?rffy |
| Author | Pascale A. Cohen |
| Abstract | Oncogene alternative splicing events can create distinct functional transcripts that offer new candidate prognostic biomarkers for breast cancer. ZNF217 is a well-established oncogene but its exon 4-skipping isoform (ZNF217-?E4) has never been investigated in terms of clinical or biological relevance. Using in silico RNA-seq and RT-qPCR analyses, we demonstrated for the first time the existence of ZNF217-?E4 transcripts in primary breast tumors, and a positive correlation between ZNF217-?E4 mRNA levels and those of the wild-type oncogene (ZNF217-WT). A pilot retrospective analysis revealed that, in the Luminal subclass, the combination of the two ZNF217 variants (the ZNF217-?E4-WT gene-expression signature) provided more information than the mRNA expression levels of each isoform alone. Ectopic overexpression of ZNF217-?E4 in breast cancer cells promoted an aggressive phenotype and an increase in ZNF217-WT expression levels that was inversely correlated with DNA methylation of the ZNF217 gene. This study provides new insights into the possible role of the ZNF217-?E4 splice variant in breast cancer and suggests a close interplay between the ZNF217-WT and ZNF217-?E4 isoforms. Our data suggest that a dual signature combining the expression levels of these two isoforms may serve as a novel prognostic biomarker allowing better stratification of breast cancers with good prognosis and aiding clinicians in therapeutic decisions. |
| Publication | Frontiers in Oncology |
| Volume | 11 |
| Pages | 647269 |
| Date | 2021 |
| Journal Abbr | Front Oncol |
| Language | eng |
| DOI | 10.3389/fonc.2021.647269 |
| ISSN | 2234-943X |
| Library Catalog | PubMed |
| Extra | PMID: 34277402 PMCID: PMC8283766 |
| Tags | breast cancer, clinic, isoform, splice variant, ZNF217 |
| Date Added | 2023/11/20 - 17:10:00 |
| Date Modified | 2023/11/20 - 17:13:10 |
| Notes and Attachments | PubMed entry (Attachment) Texte intégral (Attachment) |
Institut de Recherche en Cancérologie de
