| Added by | celine.gongora |
|---|---|
| Group name | EquipeCG |
| Item Type | Journal Article |
| Title | Anticancer Drugs for Intra-Arterial Treatment of Colorectal Cancer Liver Metastases: In-Vitro Screening after Short Exposure Time |
| Creator | Fohlen et al. |
| Author | Audrey Fohlen |
| Author | Karim Bordji |
| Author | Eric Assenat |
| Author | Céline Gongora |
| Author | Céline Bazille |
| Author | Jérémy Boulonnais |
| Author | Mikaël Naveau |
| Author | Cécile Breuil |
| Author | Elodie A. Pérès |
| Author | Myriam Bernaudin |
| Author | Boris Guiu |
| Abstract | To treat colorectal liver metastases, intra-arterial chemotherapies may complete therapeutic arsenal. Drugs using intra-arterially are very heterogeneous. The aim of this study was to select the most efficient drug on a panel of colorectal cancer (CRC) cell lines (Caco-2, HCT 116, HT 29, SW 48, SW 480, SW 620) exposed for 30 min to 12 cytotoxic agents (doxorubicin, epirubicin, idarubicin, 5-FU, raltitrexed, gemcitabine, cisplatin, oxaliplatin, mitomycin C, irinotecan, streptozocin, paclitaxel) at different concentrations. The effect on cell viability was measured using the WST-1 cell viability assay. For each drug and cell line, the IC50 and IC90 were calculated, which respectively correspond to the drug concentration (mg/mL) required to obtain 50% and 90% of cell death. We also quantified the cytotoxic index (CyI90 = C Max/IC90) to compare drug efficacy. The main findings of this study are that idarubicin emerged as the most cytotoxic agent to most of the tested CRC cell lines (Caco-2, HT29, HCT116, SW620 and SW480). Gemcitabine seemed to be the most efficient chemotherapy for SW48. Interestingly, the most commonly used cytotoxic agents in the systemic and intra-arterial treatment of colorectal liver metastasis (CRLM) (oxaliplatin, 5-FU, irinotecan) showed very limited cytotoxicity to all the cell lines. |
| Publication | Pharmaceuticals (Basel, Switzerland) |
| Volume | 14 |
| Issue | 7 |
| Pages | 639 |
| Date | 2021-07-01 |
| Journal Abbr | Pharmaceuticals (Basel) |
| Language | eng |
| DOI | 10.3390/ph14070639 |
| ISSN | 1424-8247 |
| Short Title | Anticancer Drugs for Intra-Arterial Treatment of Colorectal Cancer Liver Metastases |
| Library Catalog | PubMed |
| Extra | PMID: 34358065 PMCID: PMC8308869 |
| Tags | in vitro screening, liver neoplasm, original |
| Date Added | 2021/08/27 - 17:06:54 |
| Date Modified | 2022/08/29 - 16:31:11 |
| Notes and Attachments | PubMed entry (Attachment) Texte intégral (Attachment) |
Institut de Recherche en Cancérologie de
