| Added by | mollevi |
|---|---|
| Last modified by | jacques.colinge |
| Group name | EquipeJC |
| Item Type | Journal Article |
| Title | Proteome-wide drug and metabolite interaction mapping by thermal-stability profiling |
| Creator | Huber et al. |
| Author | K. V. Huber |
| Author | K. M. Olek |
| Author | A. C. Muller |
| Author | C. S. Tan |
| Author | K. L. Bennett |
| Author | J. Colinge |
| Author | G. Superti-Furga |
| Abstract | Thermal stabilization of proteins after ligand binding provides an efficient means to assess the binding of small molecules to proteins. We show here that in combination with quantitative mass spectrometry, the approach allows for the systematic survey of protein engagement by cellular metabolites and drugs. We profiled the targets of the drugs methotrexate and (S)-crizotinib and the metabolite 2'3'-cGAMP in intact cells and identified the 2'3'-cGAMP cognate transmembrane receptor STING, involved in immune signaling. |
| Publication | Nat Methods |
| Volume | 12 |
| Pages | 1055-7 |
| Date | Nov 2015 |
| Journal Abbr | Nature methods |
| DOI | 10.1038/nmeth.3590 |
| ISSN | 1548-7105 (Electronic) 1548-7091 (Linking) |
| Tags | corresponding, original |
| Date Added | 2018/11/14 - 11:48:35 |
| Date Modified | 2020/04/08 - 16:18:10 |
| Notes and Attachments | (Note) (Note) 26389571 (Attachment) |
Institut de Recherche en Cancérologie de
