| Added by | standudu |
|---|---|
| Group name | EquipeCTCS |
| Item Type | Journal Article |
| Title | A predictable conserved DNA base composition signature defines human core DNA replication origins |
| Creator | Akerman et al. |
| Author | Ildem Akerman |
| Author | Bahar Kasaai |
| Author | Alina Bazarova |
| Author | Pau Biak Sang |
| Author | Isabelle Peiffer |
| Author | Marie Artufel |
| Author | Romain Derelle |
| Author | Gabrielle Smith |
| Author | Marta Rodriguez-Martinez |
| Author | Manuela Romano |
| Author | Sandrina Kinet |
| Author | Peter Tino |
| Author | Charles Theillet |
| Author | Naomi Taylor |
| Author | Benoit Ballester |
| Author | Marcel Méchali |
| Abstract | DNA replication initiates from multiple genomic locations called replication origins. In metazoa, DNA sequence elements involved in origin specification remain elusive. Here, we examine pluripotent, primary, differentiating, and immortalized human cells, and demonstrate that a class of origins, termed core origins, is shared by different cell types and host ~80% of all DNA replication initiation events in any cell population. We detect a shared G-rich DNA sequence signature that coincides with most core origins in both human and mouse genomes. Transcription and G-rich elements can independently associate with replication origin activity. Computational algorithms show that core origins can be predicted, based solely on DNA sequence patterns but not on consensus motifs. Our results demonstrate that, despite an attributed stochasticity, core origins are chosen from a limited pool of genomic regions. Immortalization through oncogenic gene expression, but not normal cellular differentiation, results in increased stochastic firing from heterochromatin and decreased origin density at TAD borders. |
| Publication | Nature Communications |
| Volume | 11 |
| Issue | 1 |
| Pages | 4826 |
| Date | 2020-09-21 |
| Journal Abbr | Nat Commun |
| Language | eng |
| DOI | 10.1038/s41467-020-18527-0 |
| ISSN | 2041-1723 |
| Library Catalog | PubMed |
| Extra | PMID: 32958757 PMCID: PMC7506530 |
| Tags | Animals, Base Composition, Base Sequence, Carcinogenesis, Cell Differentiation, Cells, Cultured, DNA, DNA Replication, Genome, Human, Heterochromatin, Humans, Mice, Nucleotide Motifs, original, Replication Origin, Transcription, Genetic |
| Date Added | 2023/11/14 - 15:18:13 |
| Date Modified | 2023/11/14 - 15:46:18 |
| Notes and Attachments | PubMed entry (Attachment) Texte intégral (Attachment) |
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