| Added by | pcoopman |
|---|---|
| Group name | EquipePC |
| Item Type | Journal Article |
| Title | Combining laser capture microdissection and proteomics reveals an active translation machinery controlling invadosome formation |
| Creator | Ezzoukhry et al. |
| Author | Zakaria Ezzoukhry |
| Author | Elodie Henriet |
| Author | Fabrice P. Cordelières |
| Author | Jean-William Dupuy |
| Author | Marlène Maître |
| Author | Nathan Gay |
| Author | Sylvaine Di-Tommaso |
| Author | Luc Mercier |
| Author | Jacky G. Goetz |
| Author | Marion Peter |
| Author | Frédéric Bard |
| Author | Violaine Moreau |
| Author | Anne-Aurélie Raymond |
| Author | Frédéric Saltel |
| Abstract | Invadosomes are F-actin-based structures involved in extracellular matrix degradation, cell invasion, and metastasis formation. Analyzing their proteome is crucial to decipher their molecular composition, to understand their mechanisms, and to find specific elements to target them. However, the specific analysis of invadosomes is challenging, because it is difficult to maintain their integrity during isolation. In addition, classical purification methods often suffer from contaminations, which may impair data validation. To ensure the specific identification of invadosome components, we here develop a method that combines laser microdissection and mass spectrometry, enabling the analysis of subcellular structures in their native state based on low amounts of input material. Using this combinatorial method, we show that invadosomes contain specific components of the translational machinery, in addition to known marker proteins. Moreover, functional validation reveals that protein translation activity is an inherent property of invadosomes, which is required to maintain invadosome structure and activity. |
| Publication | Nature Communications |
| Volume | 9 |
| Issue | 1 |
| Pages | 2031 |
| Date | 2018-05-23 |
| Journal Abbr | Nat Commun |
| Language | eng |
| DOI | 10.1038/s41467-018-04461-9 |
| ISSN | 2041-1723 |
| Library Catalog | PubMed |
| Extra | PMID: 29795195 PMCID: PMC5966458 |
| Tags | Actins, Animals, Biomarkers, Tumor, Cell Line, Tumor, Chromatography, High Pressure Liquid, Humans, Laser Capture Microdissection, Mice, Neoplasms, NIH 3T3 Cells, original, Podosomes, Protein Biosynthesis, RNA, Messenger, Tandem Mass Spectrometry |
| Date Added | 2023/11/15 - 18:46:42 |
| Date Modified | 2023/11/15 - 18:47:29 |
| Notes and Attachments | PubMed entry (Attachment) Texte intégral (Attachment) |
Institut de Recherche en Cancérologie de
