| Added by | lklinares |
|---|---|
| Group name | EquipeLL |
| Item Type | Journal Article |
| Title | Glycogen metabolism is dispensable for tumour progression in clear cell renal cell carcinoma |
| Creator | Xie et al. |
| Author | Hong Xie |
| Author | Jun Song |
| Author | Jason Godfrey |
| Author | Romain Riscal |
| Author | Nicolas Skuli |
| Author | Itzhak Nissim |
| Author | M. Celeste Simon |
| Abstract | Glycogen accumulation is a highly consistent, distinguishable characteristic of clear cell renal cell carcinoma (ccRCC)1. While elevated glycogen pools might be advantageous for ccRCC cells in nutrient-deprived microenvironments to sustain tumour viability, data supporting a biological role for glycogen in ccRCC are lacking. Here, we demonstrate that glycogen metabolism is not required for ccRCC proliferation in vitro nor xenograft tumour growth in vivo. Disruption of glycogen synthesis by CRISPR-mediated knockout of glycogen synthase 1 (GYS1) has no effect on proliferation in multiple cell lines, regardless of glucose concentrations or oxygen levels. Similarly, prevention of glycogen breakdown by deletion or pharmacological inhibition of glycogen phosphorylase B (PYGB) and L (PYGL) has no impact on cell viability under any condition tested. Lastly, in vivo xenograft experiments using the ccRCC cell line, UMRC2, reveal no substantial changes in tumour size or volume when glycogen metabolism is altered, largely mimicking the phenotype of our in vitro observations. Our findings suggest that glycogen build-up in established ccRCC tumour cells is likely to be a secondary, and apparently dispensable, consequence of constitutively active hypoxia-inducible factor 1-alpha (HIF-1?) signalling. |
| Publication | Nature Metabolism |
| Volume | 3 |
| Issue | 3 |
| Pages | 327-336 |
| Date | 2021-03 |
| Journal Abbr | Nat Metab |
| Language | eng |
| DOI | 10.1038/s42255-021-00367-x |
| ISSN | 2522-5812 |
| Library Catalog | PubMed |
| Extra | PMID: 33758423 PMCID: PMC7995639 |
| Tags | Carcinoma, Renal Cell, Cell Proliferation, clinic, Disease Progression, Gene Expression Regulation, Neoplastic, Glycogen, Glycogen Synthase, Humans, Kidney Neoplasms, Tumor Microenvironment |
| Date Added | 2024/12/03 - 09:12:56 |
| Date Modified | 2024/12/03 - 09:12:56 |
| Notes and Attachments | PubMed entry (Attachment) Version acceptée (Attachment) |
Institut de Recherche en Cancérologie de
