| Added by | eric_julien |
|---|---|
| Group name | EquipeEJ |
| Item Type | Journal Article |
| Title | A switch in mitotic histone H4 lysine 20 methylation status is linked to M phase defects upon loss of HCF-1 |
| Creator | Julien and Herr |
| Author | Eric Julien |
| Author | Winship Herr |
| Abstract | The abundant chromatin-associated human factor HCF-1 is a heterodimeric complex of HCF-1N and HCF-1C subunits that are essential for two stages of the cell cycle. The HCF-1N subunit promotes G1 phase progression, whereas the HCF-1C subunit ensures proper cytokinesis at completion of M phase. How the HCF-1C subunit functions is unknown. Here, we show that HCF-1C subunit depletion causes extensive mitotic defects, including a switch from monomethyl to dimethyl lysine 20 of histone H4 (H4-K20) and defective chromosome alignment and segregation. Consistent with these activities, the HCF-1C subunit can associate with chromatin independently of the HCF-1N subunit and regulates the expression of the H4-K20 methyltransferase PR-Set7. Indeed, upregulation of PR-Set7 expression upon loss of HCF-1 leads to improper mitotic H4-K20 methylation and cytokinesis defects. These results establish the HCF-1C subunit as an important M phase regulator and suggest that H4-K20 methylation status contributes to chromosome behavior during mitosis and proper cytokinesis. |
| Publication | Molecular Cell |
| Volume | 14 |
| Issue | 6 |
| Pages | 713-725 |
| Date | Jun 18, 2004 |
| Journal Abbr | Mol. Cell |
| Language | eng |
| DOI | 10.1016/j.molcel.2004.06.008 |
| ISSN | 1097-2765 |
| Library Catalog | PubMed |
| Extra | PMID: 15200950 |
| Tags | Cell Division, Cell Line, Tumor, Chromatin, Cytokinesis, first-last-corresponding, HeLa Cells, Histone-Lysine N-Methyltransferase, Histones, Host Cell Factor C1, Humans, Lysine, Methylation, original, Protein Methyltransferases, RNA, Messenger, RNA, Small Interfering, Transcription Factors, Up-Regulation |
| Date Added | 2018/09/26 - 15:49:58 |
| Date Modified | 2019/05/28 - 13:35:27 |
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