| Added by | pcoopman |
|---|---|
| Group name | EquipePC |
| Item Type | Journal Article |
| Title | Phosphorylated Tyr142 ?-catenin localizes to centrosomes and is regulated by Syk |
| Creator | Bhardwaj et al. |
| Author | Deepshikha Bhardwaj |
| Author | Mireia Náger |
| Author | Anna Visa |
| Author | Marta C. Sallán |
| Author | Peter J. Coopman |
| Author | Carles Cantí |
| Author | Judit Herreros |
| Abstract | ?-catenin is a central component of adherent junctions and a key effector of canonical Wnt signaling, in which dephosphorylated Ser/Thr ?-catenin regulates gene transcription. ?-catenin phosphorylation at Tyr142 (PTyr142 ?-catenin), which is induced by receptor and Src family Tyr kinases, represents a previously described ?-catenin switch from adhesive to migratory roles. In addition to classical ?-catenin roles, phosphorylated Ser/Thr ?-catenin and total ?-catenin were involved in centrosomal functions, including mitotic spindle formation and centrosome separation. Here we find that PTyr142 ?-catenin is present in centrosomes in non-transformed and glioblastoma cells and that, in contrast to the Ser/Thr phosphorylated ?-catenin, PTyr142 ?-catenin centrosomal levels drop in mitosis. Furthermore, we show that the inhibitor of Spleen Tyrosine Kinase (Syk) piceatannol decreases centrosomal PTyr142 ?-catenin levels, indicating that Syk regulates centrosome PTyr142 ?-catenin. Our findings suggest that PTyr142 ?-catenin and Syk may regulate centrosomal cohesion. This study highlights the contribution of different phosphorylated ?-catenin forms to the cell and centrosome cycles. |
| Publication | Journal of Cellular Biochemistry |
| Volume | 119 |
| Issue | 4 |
| Pages | 3632-3640 |
| Date | 04 2018 |
| Journal Abbr | J. Cell. Biochem. |
| Language | eng |
| DOI | 10.1002/jcb.26571 |
| ISSN | 1097-4644 |
| Library Catalog | PubMed |
| Extra | PMID: 29231998 |
| Tags | ?-catenin, Animals, beta Catenin, Blotting, Western, Cell Line, Tumor, Cells, Cultured, Centrosome, glioblastoma, Humans, Immunoprecipitation, Mice, original, Phosphorylation, piceatannol, syk, Syk Kinase, Tubulin |
| Date Added | 2018/11/14 - 12:52:12 |
| Date Modified | 2019/05/16 - 15:24:20 |
| Notes and Attachments | PubMed entry (Attachment) |
Institut de Recherche en Cancérologie de
