| Added by | JPPOUGET |
|---|---|
| Group name | EquipeJPP |
| Item Type | Journal Article |
| Title | Targeted Radionuclide Therapy Using Auger Electron Emitters: The Quest for the Right Vector and the Right Radionuclide |
| Creator | Idrissou et al. |
| Author | Malick Bio Idrissou |
| Author | Alexandre Pichard |
| Author | Bryan Tee |
| Author | Tibor Kibedi |
| Author | Sophie Poty |
| Author | Jean-Pierre Pouget |
| Abstract | Auger electron emitters (AEEs) are attractive tools in targeted radionuclide therapy to specifically irradiate tumour cells while sparing healthy tissues. However, because of their short range, AEEs need to be brought close to sensitive targets, particularly nuclear DNA, and to a lower extent, cell membrane. Therefore, radioimmunoconjugates (RIC) have been developed for specific tumour cell targeting and transportation to the nucleus. Herein, we assessed, in A-431CEA-luc and SK-OV-31B9 cancer cells that express low and high levels of HER2 receptors, two 111In-RIC consisting of the anti-HER2 antibody trastuzumab conjugated to NLS or TAT peptides for nuclear delivery. We found that NLS and TAT peptides improved the nuclear uptake of 111In-trastuzumab conjugates, but this effect was limited and non-specific. Moreover, it did not result in a drastic decrease of clonogenic survival. Indium-111 also contributed to non-specific cytotoxicity in vitro due to conversion electrons (30% of the cell killing). Comparison with [125I]I-UdR showed that the energy released in the cell nucleus by increasing the RIC's nuclear uptake or by choosing an AEE that releases more energy per decay should be 5 to 10 times higher to observe a significant therapeutic effect. Therefore, new Auger-based radiopharmaceuticals need to be developed. |
| Publication | Pharmaceutics |
| Volume | 13 |
| Issue | 7 |
| Pages | 980 |
| Date | 2021-06-29 |
| Journal Abbr | Pharmaceutics |
| Language | eng |
| DOI | 10.3390/pharmaceutics13070980 |
| ISSN | 1999-4923 |
| Short Title | Targeted Radionuclide Therapy Using Auger Electron Emitters |
| Library Catalog | PubMed |
| Extra | Number: 7 PMID: 34209637 PMCID: PMC8309076 |
| Tags | Auger electrons, first-last-corresponding, NLS peptide, nuclear localisation sequence, original, radionuclide therapy, Scientific, TAT peptide, top |
| Date Added | 2023/11/23 - 12:44:38 |
| Date Modified | 2024/12/15 - 12:39:05 |
| Notes and Attachments | PubMed entry (Attachment) Texte intégral (Attachment) |
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