| Added by | mollevi |
|---|---|
| Last modified by | llasorsa |
| Group name | EquipeMY |
| Item Type | Journal Article |
| Title | Hypoxia differently modulates the release of mitochondrial and nuclear DNA |
| Creator | Otandault et al. |
| Author | Jean-Daniel Abraham |
| Author | Zahra Al Amir Dache |
| Author | Abdelnaby Khalyfa |
| Author | Isabelle Jariel-Encontre |
| Author | Thierry Forné |
| Author | Corinne Prévostel |
| Author | Salem Chouaib |
| Author | David Gozal |
| Author | Alain R. Thierry |
| Abstract | BACKGROUND: We investigated the influence of hypoxia on the concentration of mitochondrial and nuclear cell-free DNA (McfDNA and NcfDNA, respectively). METHOD: By an ultra-sensitive quantitative PCR-based assay, McfDNA and NcfDNA were measured in the supernatants of different colorectal cell lines, and in the plasma of C57/Bl6 mice engrafted with TC1 tumour cells, in normoxic or hypoxic conditions. RESULTS: Our data when setting cell culture conditions highlighted the higher stability of McfDNA as compared to NcfDNA and revealed that cancer cells released amounts of nuclear DNA equivalent to the mass of a chromosome over a 6-h duration of incubation. In cell model, hypoxia induced a great increase in NcfDNA and McfDNA concentrations within the first 24?h. After this period, cfDNA total concentrations remained stable in hypoxia consecutive to a decrease of nuclear DNA release, and noteworthy, to a complete inhibition of daily mitochondrial DNA release. In TC1-engrafted mice submitted to intermittent hypoxia, plasma NcfDNA levels are much higher than in mice bred in normoxia, unlike plasma McfDNA concentration that is not impacted by hypoxia. CONCLUSION: This study suggests that hypoxia negatively modulates nuclear and, particularly, mitochondrial DNA releases in long-term hypoxia, and revealed that the underlying mechanisms are differently regulated. |
| Publication | British Journal of Cancer |
| Date | Jan 13, 2020 |
| Journal Abbr | Br. J. Cancer |
| Language | eng |
| DOI | 10.1038/s41416-019-0716-y |
| ISSN | 1532-1827 |
| Library Catalog | PubMed |
| Extra | PMID: 31929518 |
| Tags | Animals, Cell Line, Tumor, Cell Nucleus, Colorectal Neoplasms, DNA, Mitochondrial, HCT116 Cells, Humans, Male, Mice, Mice, Inbred C57BL, Tumor Hypoxia |
| Date Added | 2020/01/28 - 09:46:11 |
| Date Modified | 2024/10/10 - 16:57:59 |
| Notes and Attachments | PubMed entry (Attachment) PubMed entry (Attachment) Texte intégral (Attachment) Texte intégral (Attachment) Texte intégral (Attachment) Texte intégral (Attachment) |
Institut de Recherche en Cancérologie de
