| Added by | pcoopman |
|---|---|
| Group name | EquipePC |
| Item Type | Journal Article |
| Title | GOLT1B Activation in Hepatitis C Virus-Infected Hepatocytes Links ER Trafficking and Viral Replication |
| Creator | Butterworth et al. |
| Author | Jacqueline Butterworth |
| Author | Damien Gregoire |
| Author | Marion Peter |
| Author | Armando Andres Roca Suarez |
| Author | Guillaume Desandré |
| Author | Yannick Simonin |
| Author | Alessia Virzě |
| Author | Amal Zine El Aabidine |
| Author | Marine Guivarch |
| Author | Jean-Christophe Andrau |
| Author | Edouard Bertrand |
| Author | Eric Assenat |
| Author | Joachim Lupberger |
| Author | Urszula Hibner |
| Abstract | Chronic hepatitis C carries a high risk of development of hepatocellular carcinoma (HCC), triggered by both direct and indirect effects of the virus. We examined cell-autonomous alterations in gene expression profiles associated with hepatitis C viral presence. Highly sensitive single molecule fluorescent in situ hybridization applied to frozen tissue sections of a hepatitis C patient allowed the delineation of clusters of infected hepatocytes. Laser microdissection followed by RNAseq analysis of hepatitis C virus (HCV)-positive and -negative regions from the tumoral and non-tumoral tissues from the same patient revealed HCV-related deregulation of expression of genes in the tumor and in the non-tumoral tissue. However, there was little overlap between both gene sets. Our interest in alterations that increase the probability of tumorigenesis prompted the examination of genes whose expression was increased by the virus in the non-transformed cells and whose level remained high in the tumor. This strategy led to the identification of a novel HCV target gene: GOLT1B, which encodes a protein involved in ER-Golgi trafficking. We further show that GOLT1B expression is induced during the unfolded protein response, that its presence is essential for efficient viral replication, and that its expression is correlated with poor outcome in HCC. |
| Publication | Pathogens (Basel, Switzerland) |
| Volume | 11 |
| Issue | 1 |
| Pages | 46 |
| Date | 2021-12-31 |
| Journal Abbr | Pathogens |
| Language | eng |
| DOI | 10.3390/pathogens11010046 |
| ISSN | 2076-0817 |
| Library Catalog | PubMed |
| Extra | PMID: 35055994 PMCID: PMC8781247 |
| Tags | hepatitis C, hepatocellular carcinoma, original, smiFISH, transcriptomic profiling |
| Date Added | 2023/11/15 - 18:13:01 |
| Date Modified | 2023/11/15 - 18:13:18 |
| Notes and Attachments | PubMed entry (Attachment) Texte intégral (Attachment) |
Institut de Recherche en Cancérologie de
