Added by |
JPPOUGET |
Group name |
EquipeJPP |
Item Type |
Journal Article |
Title |
Clonogenic assay to measure bystander cytotoxicity of targeted alpha-particle therapy |
Creator |
Constanzo et al. |
Author |
Julie Constanzo |
Author |
Clara Diaz Garcia-Prada |
Author |
Jean-Pierre Pouget |
Abstract |
Radiation therapy induces targeted effects in the cells that are irradiated and also non-targeted effects (i.e. bystander effects) in non-irradiated cells that are close to or at short distance (1 mm) from irradiated cells. Bystander effects are mediated by intercellular communications and may result in cytotoxic and genotoxic modifications. Their occurrence and relative contribution to the irradiation outcome are influenced by several parameters among which the particle linear energy transfer seems to be prominent. Bystander effects were first observed after external radiation therapy, but have been described also following targeted radionuclide therapy. Therefore, we propose a method to investigate their occurrence in experimental conditions where cells are exposed to radiopharmaceuticals. In this approach, clonogenic cell death is the biological endpoint of the bystander effects caused by irradiation with alpha particles (a potent inducer of the bystander response). |
Publication |
Methods in Cell Biology |
Volume |
174 |
Pages |
137-149 |
Date |
2023 |
Journal Abbr |
Methods Cell Biol |
Language |
eng |
DOI |
10.1016/bs.mcb.2022.08.005 |
ISSN |
0091-679X |
Library Catalog |
PubMed |
Extra |
PMID: 36710047 |
Tags |
Alpha Particles, Bystander Effect, Castration-resistant prostate cancer, Cell Communication, Cell Line, Tumor, Colony formation assay, first-last-coresponding, Methodology, Xofigo? |
Date Added |
2024/12/07 - 07:04:20 |
Date Modified |
2024/12/15 - 12:10:01 |
Notes and Attachments |
PubMed entry (Attachment) |