| Added by | alainmange |
|---|---|
| Group name | PlateformePP2I |
| Item Type | Journal Article |
| Title | Development of Amino Acids Functionalized SBA-15 for the Improvement of Protein Adsorption |
| Creator | Gutiérrez-Climente et al. |
| Author | Raquel Gutiérrez-Climente |
| Author | Margaux Clavié |
| Author | Jérémie Gouyon |
| Author | Giang Ngo |
| Author | Yoann Ladner |
| Author | Pascal Etienne |
| Author | Pascal Dumy |
| Author | Pierre Martineau |
| Author | Martine Pugnière |
| Author | Catherine Perrin |
| Author | Gilles Subra |
| Author | Ahmad Mehdi |
| Abstract | Ordered mesoporous materials and their modification with multiple functional groups are of wide scientific interest for many applications involving interaction with biological systems and biomolecules (e.g., catalysis, separation, sensor design, nano-science or drug delivery). In particular, the immobilization of enzymes onto solid supports is highly attractive for industry and synthetic chemistry, as it allows the development of stable and cheap biocatalysts. In this context, we developed novel silylated amino acid derivatives (Si-AA-NH2) that have been immobilized onto SBA-15 materials in biocompatible conditions avoiding the use of toxic catalyst, solvents or reagents. The resulting amino acid-functionalized materials (SBA-15@AA) were characterized by XRD, TGA, EA, Zeta potential, nitrogen sorption and FT-IR. Differences of the physical properties (e.g., charges) were observed while the structural ones remained unchanged. The adsorption of the enzyme lysozyme (Lyz) onto the resulting functionalized SBA-15@AA materials was evaluated at different pHs. The presence of different functional groups compared with bare SBA-15 showed better adsorption results, for example, 79.6 nmol of Lyz adsorbed per m2 of SBA-15@Tyr compared with the 44.9 nmol/m2 of the bare SBA-15. |
| Publication | Molecules (Basel, Switzerland) |
| Volume | 26 |
| Issue | 19 |
| Pages | 6085 |
| Date | 2021-10-08 |
| Journal Abbr | Molecules |
| Language | eng |
| DOI | 10.3390/molecules26196085 |
| ISSN | 1420-3049 |
| Library Catalog | PubMed |
| Extra | PMID: 34641630 PMCID: PMC8512485 |
| Tags | Adsorption, Amino Acids, author, Enzymes, Immobilized, Hydrogen-Ion Concentration, lysozyme, Molecular Structure, Muramidase, original, Porosity, pp2i, SBA-15, Silicon Dioxide, silylated amino acids, sol - gel, Spectroscopy, Fourier Transform Infrared, Surface Properties |
| Date Added | 2021/12/09 - 10:06:49 |
| Date Modified | 2021/12/09 - 10:07:52 |
| Notes and Attachments | PubMed entry (Attachment) |
Institut de Recherche en Cancérologie de
