| Added by | eric_julien |
|---|---|
| Group name | EquipeEJ |
| Item Type | Journal Article |
| Title | Epigenetic regulation of histone H3 serine 10 phosphorylation status by HCF-1 proteins in C. elegans and mammalian cells |
| Creator | Lee et al. |
| Author | Soyoung Lee |
| Author | Virginie Horn |
| Author | Eric Julien |
| Author | Yi Liu |
| Author | Joanna Wysocka |
| Author | Bruce Bowerman |
| Author | Michael O. Hengartner |
| Author | Winship Herr |
| Abstract | BACKGROUND: The human herpes simplex virus (HSV) host cell factor HCF-1 is a transcriptional coregulator that associates with both histone methyl- and acetyltransferases, and a histone deacetylase and regulates cell proliferation and division. In HSV-infected cells, HCF-1 associates with the viral protein VP16 to promote formation of a multiprotein-DNA transcriptional activator complex. The ability of HCF proteins to stabilize this VP16-induced complex has been conserved in diverse animal species including Drosophila melanogaster and Caenorhabditis elegans suggesting that VP16 targets a conserved cellular function of HCF-1. METHODOLOGY/PRINCIPAL FINDINGS: To investigate the role of HCF proteins in animal development, we have characterized the effects of loss of the HCF-1 homolog in C. elegans, called Ce HCF-1. Two large hcf-1 deletion mutants (pk924 and ok559) are viable but display reduced fertility. Loss of Ce HCF-1 protein at reduced temperatures (e.g., 12 degrees C), however, leads to a high incidence of embryonic lethality and early embryonic mitotic and cytokinetic defects reminiscent of mammalian cell-division defects upon loss of HCF-1 function. Even when viable, however, at normal temperature, mutant embryos display reduced levels of phospho-histone H3 serine 10 (H3S10P), a modification implicated in both transcriptional and mitotic regulation. Mammalian cells with defective HCF-1 also display defects in mitotic H3S10P status. CONCLUSIONS/SIGNIFICANCE: These results suggest that HCF-1 proteins possess conserved roles in the regulation of cell division and mitotic histone phosphorylation. |
| Publication | PloS One |
| Volume | 2 |
| Issue | 11 |
| Pages | e1213 |
| Date | Nov 28, 2007 |
| Journal Abbr | PLoS ONE |
| Language | eng |
| DOI | 10.1371/journal.pone.0001213 |
| ISSN | 1932-6203 |
| Library Catalog | PubMed |
| Extra | PMID: 18043729 PMCID: PMC2082077 |
| Tags | Animals, Base Sequence, Caenorhabditis elegans, Caenorhabditis elegans Proteins, DNA Primers, Epigenesis, Genetic, Histones, Host Cell Factor C1, Humans, Microscopy, Fluorescence, Mutation, Phosphorylation, Serine |
| Date Added | 2018/09/26 - 15:50:04 |
| Date Modified | 2018/09/26 - 15:50:04 |
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