| Added by | standudu |
|---|---|
| Group name | EquipeAT |
| Item Type | Journal Article |
| Title | Accessibilome of human glioblastoma: collagen-VI-alpha-1 is a new target and a marker of poor outcome |
| Creator | Turtoi et al. |
| Author | Andrei Turtoi |
| Author | Arnaud Blomme |
| Author | Elettra Bianchi |
| Author | Pamela Maris |
| Author | Riccardo Vannozzi |
| Author | Antonio Giuseppe Naccarato |
| Author | Philippe Delvenne |
| Author | Edwin De Pauw |
| Author | Generoso Bevilacqua |
| Author | Vincent Castronovo |
| Abstract | Functional targeted therapy has unfortunately failed to improve the outcome of glioblastoma patients. Success stories evidenced by the use of antibody-drug conjugates in other tumor types are encouraging, but targets specific to glioblastoma and accessible through the bloodstream remain scarce. In the current work, we have identified and characterized novel and accessible proteins using an innovative proteomic approach on six human glioblastomas; the corresponding data have been deposited in the PRIDE database identifier PXD001398. Among several clusters of uniquely expressed proteins, we highlight collagen-VI-alpha-1 (COL6A1) as a highly expressed tumor biomarker with low levels in most normal tissues. Immunohistochemical analysis of glioma samples from 61 patients demonstrated that COL6A1 is a significant and consistent feature of high-grade glioma. Deposits of COL6A1 were evidenced in the perivascular regions of the tumor-associated vasculature and in glioma cells found in pseudopalisade structures. Retrospective analysis of public gene-expression data sets from over 300 glioma patients demonstrated a significant correlation of poor patient outcome and high COL6A1 expression. In a proof-of-concept study, we use chicken chorioallantoic membrane in vivo model to show that COL6A1 is a reachable target for IV-injected antibodies. The present data warrant further development of human COL6A1 antibodies for assessing the quantitative biodistribution in the preclinical tumor models. |
| Publication | Journal of Proteome Research |
| Volume | 13 |
| Issue | 12 |
| Pages | 5660-5669 |
| Date | Dec 05, 2014 |
| Journal Abbr | J. Proteome Res. |
| Language | eng |
| DOI | 10.1021/pr500657w |
| ISSN | 1535-3907 |
| Short Title | Accessibilome of human glioblastoma |
| Library Catalog | PubMed |
| Extra | PMID: 25325876 |
| Tags | angiogenesis, Animals, Biomarkers, Tumor, Blotting, Western, Brain Neoplasms, Cell Line, Tumor, Chick Embryo, Chorioallantoic Membrane, Chromatography, High Pressure Liquid, Collagen Type VI, first-last-corresponding, Humans, Immunohistochemistry, Kaplan-Meier Estimate, Microscopy, Fluorescence, original, perivascular, Prognosis, Tandem Mass Spectrometry, targeted therapy, Transplantation, Heterologous |
| Date Added | 2019/05/29 - 14:24:01 |
| Date Modified | 2019/05/29 - 15:50:17 |
| Notes and Attachments | PubMed entry (Attachment) Texte intégral (Attachment) |
Institut de Recherche en Cancérologie de
