| Added by | mollevi |
|---|---|
| Last modified by | jacques.colinge |
| Group name | EquipeJC |
| Item Type | Journal Article |
| Title | A Comprehensive Analysis of the Dynamic Response to Aphidicolin-Mediated Replication Stress Uncovers Targets for ATM and ATMIN |
| Creator | Mazouzi et al. |
| Author | A. Mazouzi |
| Author | A. Stukalov |
| Author | A. C. Muller |
| Author | D. Chen |
| Author | M. Wiedner |
| Author | J. Prochazkova |
| Author | S. C. Chiang |
| Author | M. Schuster |
| Author | F. P. Breitwieser |
| Author | A. Pichlmair |
| Author | S. F. El-Khamisy |
| Author | C. Bock |
| Author | R. Kralovics |
| Author | J. Colinge |
| Author | K. L. Bennett |
| Author | J. I. Loizou |
| Abstract | The cellular response to replication stress requires the DNA-damage-responsive kinase ATM and its cofactor ATMIN; however, the roles of this signaling pathway following replication stress are unclear. To identify the functions of ATM and ATMIN in response to replication stress, we utilized both transcriptomics and quantitative mass-spectrometry-based phosphoproteomics. We found that replication stress induced by aphidicolin triggered widespread changes in both gene expression and protein phosphorylation patterns. These changes gave rise to distinct early and late replication stress responses. Furthermore, our analysis revealed previously unknown targets of ATM and ATMIN downstream of replication stress. We demonstrate ATMIN-dependent phosphorylation of H2AX and of CRMP2, a protein previously implicated in Alzheimer's disease but not in the DNA damage response. Overall, our dataset provides a comprehensive resource for discovering the cellular responses to replication stress and, potentially, associated pathologies. |
| Publication | Cell Rep |
| Date | Apr 14 2016 |
| Journal Abbr | Cell reports |
| DOI | 10.1016/j.celrep.2016.03.077 |
| ISSN | 2211-1247 (Electronic) |
| Tags | original, phd, postdoc |
| Date Added | 2018/11/14 - 11:48:35 |
| Date Modified | 2019/10/22 - 21:17:47 |
| Notes and Attachments | (Note) (Note) 27149854 (Attachment) |
Institut de Recherche en Cancérologie de
