| Added by | lklinares |
|---|---|
| Group name | EquipeLL |
| Item Type | Journal Article |
| Title | Zika M Oligopeptide ZAMP Confers Cell Death-Promoting Capability to a Soluble Tumor-Associated Antigen through Caspase-3/7 Activation |
| Creator | Vanwalscappel et al. |
| Author | Bénédicte Vanwalscappel |
| Author | Juliano G. Haddad |
| Author | Roba Almokdad |
| Author | Jason Decotter |
| Author | Gilles Gadea |
| Author | Philippe Desprès |
| Abstract | Mosquito-borne Zika virus (ZIKV) is an emerging flavivirus of medical concern associated with neurological disorders. ZIKV utilizes apoptosis as a mechanism of cell killing. The structural M protein may play a role in flavivirus-induced apoptosis. The death-promoting capability of M has been restricted to an oligopeptide representing the residues M-32/40. Here, we evaluated the apoptosis inducing ability of the residues M-31/41 of ZIKV. The ZIKV M oligopeptide was associated to a soluble form of GFP (sGFP) and the resulting sGFP-M31/41 construct was assessed in Huh7 cells. Expression of sGFP-M31/41 can trigger apoptosis in Huh7 cells through caspase-3/7 activation. The translocation of sGFP-M31/41 in the endoplasmic reticulum was a prerequisite for apoptosis induction. The residues M-33/35/38 may play a critical role in the death-promoting activity of sGFP-M31/41. The effect of ZIKV M oligopeptide defined as ZAMP (for Zika Apoptosis M Peptide) on expression of a tumor-associated antigen was assayed on megakaryocyte-potentiating factor (MPF). Expression of MPF-ZAMP construct resulted in caspase-associated apoptosis activation in A549 and Huh7 cells. ZIKV has been proposed as an oncolytic virus for cancer therapy. The ability of the Zika M oligopeptide to confer death-promoting capability to MPF opens up attractive perspectives for ZAMP as an innovative anticancer agent. |
| Publication | International Journal of Molecular Sciences |
| Volume | 21 |
| Issue | 24 |
| Pages | 9578 |
| Date | 2020-12-16 |
| Journal Abbr | Int J Mol Sci |
| Language | eng |
| DOI | 10.3390/ijms21249578 |
| ISSN | 1422-0067 |
| Library Catalog | PubMed |
| Extra | PMID: 33339164 PMCID: PMC7765671 |
| Tags | A549 Cells, anticancer agent, anticancer virotherapy, Antigens, Neoplasm, Apoptosis, apoptotic cell death, arbovirus, Caspase 3, Caspase 7, caspase-3/7 activation, GPI-Linked Proteins, HEK293 Cells, Humans, M protein, Mesothelin, Oligopeptides, original, tumor cells, tumor-associated antigen, viral apoptosis inducer, Viral Matrix Proteins, viral oligopeptide |
| Date Added | 2024/12/03 - 09:12:56 |
| Date Modified | 2024/12/03 - 09:12:56 |
| Notes and Attachments | PubMed entry (Attachment) Texte intégral (Attachment) |
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