| Added by | mollevi |
|---|---|
| Group name | PlateformeRHEM |
| Item Type | Journal Article |
| Title | Lung endothelial barrier disruption in Lyl1-deficient mice |
| Creator | Pirot et al. |
| Author | Nelly Pirot |
| Author | Hélène Delpech |
| Author | Virginie Deleuze |
| Author | Christiane Dohet |
| Author | Monique Courtade-Saïdi |
| Author | Céline Basset-Léobon |
| Author | Elias Chalhoub |
| Author | Danièle Mathieu |
| Author | Valérie Pinet |
| Abstract | Maturation of newly formed vessels is a multistep phenomenon during which functional endothelial barriers are established. Disruption of vessel integrity is an important feature in many physiological and pathological processes. We previously reported that lymphoblastic leukemia-derived sequence 1 (LYL1) is required for the late stages of postnatal angiogenesis to limit the formation of new blood vessels, notably by regulating the activity of the small GTPase Rap1. In this study, we show that LYL1 is also required during the formation of the mature endothelial barrier in the lungs of adult mice. Specifically, LYL1 knockdown in human endothelial cells downregulated the expression of ARHGAP21 and ARHGAP24, which encode two Rho GTPase-activating proteins, and this was correlated with increased RhoA activity and reorganization of the actin cytoskeleton into stress fibers. Importantly, in lungs of Lyl1-deficient mice, both vascular endothelial (VE)-cadherin and p120-catenin were poorly recruited to endothelial adherens junctions, indicative of defective cell-cell junctions. Consistent with this, higher Evans blue dye extravasation, edema, and leukocyte infiltration in the lung parenchyma of Lyl1-/- mice than in wild-type littermates confirmed that lung vascular permeability is constitutively elevated in Lyl1-/- adult mice. Our data show that LYL1 acts as a stabilizing signal for adherens junction formation by operating upstream of VE-cadherin and of the two GTPases Rap1 and RhoA. As increased vascular permeability is a key feature and a major mechanism of acute respiratory distress syndrome, molecules that regulate LYL1 activity could represent additional tools to modify the endothelial barrier permeability. |
| Publication | American Journal of Physiology. Lung Cellular and Molecular Physiology |
| Volume | 306 |
| Issue | 8 |
| Pages | L775-785 |
| Date | Apr 15, 2014 |
| Journal Abbr | Am. J. Physiol. Lung Cell Mol. Physiol. |
| Language | eng |
| DOI | 10.1152/ajplung.00200.2013 |
| ISSN | 1522-1504 |
| Library Catalog | PubMed |
| Extra | PMID: 24532287 |
| Tags | Actins, Animals, Basic Helix-Loop-Helix Transcription Factors, bHLH, Blotting, Western, Cell Membrane Permeability, Cells, Cultured, Endothelium, Vascular, Fluorescent Antibody Technique, GTPase-Activating Proteins, Human Umbilical Vein Endothelial Cells, Humans, Immunoenzyme Techniques, Lung, lymphoblastic leukemia-derived sequence 1, Mice, Mice, Inbred C57BL, Mice, Knockout, Neoplasm Proteins, original, rap1 GTP-Binding Proteins, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, RhoA, rhoA GTP-Binding Protein, RNA, Messenger, Stress Fibers, vascular endothelial-cadherin, vascular permeability |
| Date Added | 2019/06/04 - 17:45:12 |
| Date Modified | 2019/06/05 - 11:08:40 |
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