| Added by | mollevi |
|---|---|
| Last modified by | alainmange |
| Group name | PlateformePP2I |
| Item Type | Journal Article |
| Title | Binding analysis between monomeric ?-casein and hydrophobic bioactive compounds investigated by surface plasmon resonance and fluorescence spectroscopy |
| Creator | Bahri et al. |
| Author | Asma Bahri |
| Author | Corinne Henriquet |
| Author | Martine Pugnière |
| Author | Sylvie Marchesseau |
| Author | Dominique Chevalier-Lucia |
| Abstract | ?-Casein, a phosphoprotein representing 37% of the bovine milk caseins, has specific features promoting its application as a nanocarrier for hydrophobic bioactives. In this study, the interactions of ?-casein with curcumin and vitamin D3 under the same physico-chemical conditions were investigated. The interaction kinetics have been studied by surface plasmon resonance (SPR) and fluorescence spectroscopy. The KD value for curcumin-?-casein interaction has been successfully evaluated (4.1?±?0.7?×?10-4?M) using SPR by fitting data to a 1:1 Langmuir interaction model. Conversely, the SPR responses obtained for vitamin D3 show that the interactions between this hydrophobic compound and the ?-casein immobilized on the sensor chip were below the sensitivity of the SPR apparatus. Moreover, the fluorescence quenching data show that curcumin has higher affinity to ?-casein (KA?=?23.5?±?1.9?×?104?M-1) than vitamin D3 (KA?=?5.8?±?1.1?×?104?M-1). |
| Publication | Food Chemistry |
| Volume | 286 |
| Pages | 289-296 |
| Date | Jul 15, 2019 |
| Journal Abbr | Food Chem |
| Language | eng |
| DOI | 10.1016/j.foodchem.2019.01.176 |
| ISSN | 1873-7072 |
| Library Catalog | PubMed |
| Extra | PMID: 30827608 |
| Tags | ?-Casein, Animals, author, Binding parameters, Caseins, Cattle, Cholecalciferol, Curcumin, Curcumin (PubChem CID 969516), Fluorescence quenching, Hydrophobic and Hydrophilic Interactions, Micelles, Milk, original, pp2i, Protein Binding, Spectrometry, Fluorescence, Surface Plasmon Resonance, Vitamin D3, Vitamin D3 (PubChem CID 5280795) |
| Date Added | 2019/06/04 - 17:17:14 |
| Date Modified | 2020/01/14 - 10:24:59 |
Institut de Recherche en Cancérologie de
