| Added by | André Pèlegrin |
|---|---|
| Group name | EquipeAP |
| Item Type | Journal Article |
| Title | Multi-centre technical evaluation of the radiation-induced lymphocyte apoptosis assay as a predictive test for radiotherapy toxicity |
| Creator | Talbot et al. |
| Author | Christopher J. Talbot |
| Author | Marlon R. Veldwijk |
| Author | David Azria |
| Author | Chiara Batini |
| Author | Miriam Bierbaum |
| Author | Muriel Brengues |
| Author | Jenny Chang-Claude |
| Author | Kerstie Johnson |
| Author | Anke Keller |
| Author | Sheila Smith |
| Author | Elena Sperk |
| Author | R. Paul Symonds |
| Author | Frederik Wenz |
| Author | Catharine M. L. West |
| Author | Carsten Herskind |
| Abstract | Predicting which patients will develop adverse reactions to radiotherapy is important for personalised treatment. Prediction will require an algorithm or nomogram combining clinical and biological data. The radiation-induced lymphocyte apoptosis (RILA) assay is the leading candidate as a biological predictor of radiotherapy toxicity. In this study we tested the potential of the assay for standardisation and use in multiple testing laboratories. The assay was standardised and reproducibility determined using samples from healthy volunteers assayed concurrently in three laboratories in Leicester (UK), Mannheim (Germany) and Montpellier (France). RILA assays were performed on samples taken prior to radiotherapy from 1319 cancer patients enrolled in the REQUITE project at multiple centres. The patients were being treated for breast (n?=?753), prostate (n?=?506) or lung (n?=?60) cancer. Inter-laboratory comparisons identified several factors affecting results: storage time, incubation periods and type of foetal calf serum. Following standardisation, there was no significant difference in results between the centres. Significant differences were seen in RILA scores between cancer types (prostate?>?breast?>?lung), by smoking status (non-smokers?>?smokers) and co-morbidity with rheumatoid arthritis (arthritics?>?non-arthritics). An analysis of acute radiotherapy toxicity showed as expected that RILA assay does not predict most end-points, but unexpectedly did predict acute breast pain. This result may elucidate the mechanism by which the RILA assay predicts late radiotherapy toxicity. The work shows clinical trials involving multiple laboratory measurement of the RILA assay are feasible and the need to account for tumour type and other variables when applying to predictive models. |
| Publication | Clinical and Translational Radiation Oncology |
| Volume | 18 |
| Pages | 1-8 |
| Date | Sep 2019 |
| Journal Abbr | Clin Transl Radiat Oncol |
| Language | eng |
| DOI | 10.1016/j.ctro.2019.06.001 |
| ISSN | 2405-6308 |
| Library Catalog | PubMed |
| Extra | 00000 PMID: 31341970 PMCID: PMC6610684 |
| Tags | clinic, collaboration, original, top |
| Date Added | 2019/09/12 - 09:51:01 |
| Date Modified | 2019/12/19 - 08:49:47 |
| Notes and Attachments | PubMed entry (Attachment) |
Institut de Recherche en Cancérologie de
