| Added by | André Pèlegrin |
|---|---|
| Group name | EquipeAP |
| Item Type | Journal Article |
| Title | Analysis of Circulating Tumor Cells in Patients with Non-Metastatic High-Risk Prostate Cancer before and after Radiotherapy Using Three Different Enumeration Assays |
| Creator | Budna-Tukan et al. |
| Author | Joanna Budna-Tukan |
| Author | Monika ?wierczewska |
| Author | Martine Mazel |
| Author | Wojciech A. Cie?likowski |
| Author | Agnieszka Ida |
| Author | Agnieszka Jankowiak |
| Author | Andrzej Antczak |
| Author | Micha? Nowicki |
| Author | Klaus Pantel |
| Author | David Azria |
| Author | Maciej Zabel |
| Author | Catherine Alix-Panabières |
| Abstract | The characterization of circulating tumor cells (CTCs) can lead to a promising strategy for monitoring residual or relapsing prostate cancer (PCa) after local therapy. The aim of this study was to compare three innovative technologies for CTC enumeration in 131 high-risk patients with PCa, before and after radiotherapy, combined with androgen deprivation. The CTC number was tested using the FDA-cleared CellSearch® system, the dual fluoro-EPISPOT assay that only detects functional CTCs, and the in vivo CellCollector® technology. The highest percentage of CTC-positive patients was detected with the CellCollector® (48%) and dual fluoro-EPISPOT (42%) assays, while the CellSearch® system presented the lowest rate (14%). Although the concordance among methods was only 23%, the cumulative positivity rate was 79%. A matched-pair analysis of the samples before, and after, treatment suggested a trend toward a decrease in CTC count after treatment with all methods. CTC tended to be positivity correlated with age for the fluoro-EPISPOT assay and with PSA level from the data of three assays. Combining different CTC assays improved CTC detection rates in patients with non-metastatic high-risk PCa before and after treatment. Our findings do not support the hypothesis that radiotherapy leads to cancer cell release in the circulation. |
| Publication | Cancers |
| Volume | 11 |
| Issue | 6 |
| Date | Jun 10, 2019 |
| Journal Abbr | Cancers (Basel) |
| Language | eng |
| DOI | 10.3390/cancers11060802 |
| ISSN | 2072-6694 |
| Library Catalog | PubMed |
| Extra | 00000 PMID: 31185699 PMCID: PMC6627099 |
| Tags | clinic, CTC, early diagnostic, liquid biopsy, nonvisible, PSA |
| Date Added | 2019/07/25 - 11:56:13 |
| Date Modified | 2019/12/11 - 18:25:40 |
| Notes and Attachments | PubMed entry (Attachment) Texte intégral (Attachment) |
Institut de Recherche en Cancérologie de
