| Added by | pmartino |
|---|---|
| Group name | EquipePM |
| Item Type | Journal Article |
| Title | Recruitment of ubiquitin-activating enzyme UBA1 to DNA by poly(ADP-ribose) promotes ATR signalling |
| Creator | Kumbhar et al. |
| Author | Ramhari Kumbhar |
| Author | Sophie Vidal-Eychenié |
| Author | Dimitrios-Georgios Kontopoulos |
| Author | Marion Larroque |
| Author | Christian Larroque |
| Author | Jihane Basbous |
| Author | Sofia Kossida |
| Author | Cyril Ribeyre |
| Author | Angelos Constantinou |
| Abstract | The DNA damage response (DDR) ensures cellular adaptation to genotoxic insults. In the crowded environment of the nucleus, the assembly of productive DDR complexes requires multiple protein modifications. How the apical E1 ubiquitin activation enzyme UBA1 integrates spatially and temporally in the DDR remains elusive. Using a human cell-free system, we show that poly(ADP-ribose) polymerase 1 promotes the recruitment of UBA1 to DNA. We find that the association of UBA1 with poly(ADP-ribosyl)ated protein-DNA complexes is necessary for the phosphorylation replication protein A and checkpoint kinase 1 by the serine/threonine protein kinase ataxia-telangiectasia and RAD3-related, a prototypal response to DNA damage. UBA1 interacts directly with poly(ADP-ribose) via a solvent-accessible and positively charged patch conserved in the Animalia kingdom but not in Fungi. Thus, ubiquitin activation can anchor to poly(ADP-ribose)-seeded protein assemblies, ensuring the formation of functional ataxia-telangiectasia mutated and RAD3-related-signalling complexes. |
| Publication | Life Science Alliance |
| Volume | 1 |
| Issue | 3 |
| Pages | e201800096 |
| Date | Jun 2018 |
| Journal Abbr | Life Sci Alliance |
| Language | eng |
| DOI | 10.26508/lsa.201800096 |
| ISSN | 2575-1077 |
| Library Catalog | PubMed |
| Extra | PMID: 30456359 PMCID: PMC6238597 |
| Tags | arc, epigenmed, original, private, siric |
| Date Added | 2018/11/29 - 10:55:50 |
| Date Modified | 2021/03/05 - 10:43:56 |
| Notes and Attachments | PubMed entry (Attachment) Texte intégral (Attachment) |
Institut de Recherche en Cancérologie de
