Added by |
standudu |
Group name |
EquipeCTCS |
Item Type |
Journal Article |
Title |
Detection of Circulating Tumor Cells in Cerebrospinal Fluid of Patients with Suspected Breast Cancer Leptomeningeal Metastases: A Prospective Study |
Creator |
Darlix et al. |
Author |
Amélie Darlix |
Author |
Laure Cayrefourcq |
Author |
Nicolas Menjot de Champfleur |
Author |
Alexis Bievelez |
Author |
William Jacot |
Author |
Cristina Leaha |
Author |
Simon Thezenas |
Author |
Catherine Alix-Panabières |
Abstract |
BACKGROUND: The diagnosis of breast cancer (BC)-related leptomeningeal metastases (LM) relies on the detection of tumor cells in cerebrospinal fluid (CSF) using conventional cytology (gold standard). However, the sensitivity of this technique is low. Our goal was to evaluate whether circulating tumor cell (CTC) detection in CSF using the CellSearch® system could be used for LM diagnosis.
METHODS: This prospective, monocentric study included adult patients with suspected BC-related LM. The clinical sensitivity and specificity of CTC detection in CSF for LM diagnosis were calculated relative to conventional CSF cytology.
RESULTS: Forty-nine eligible patients were included and 40 were evaluable (CTC detection technical failure: n?=?8, eligibility criteria failure: n?=?1). Cytology was positive in 18/40 patients. CTCs were detected in these 18 patients (median: 5824 CTC, range: 93 to 45052) and in 5/22 patients with negative cytology (median: 2 CTC, range: 1 to 44). The detection of ?1 CSF CTC was associated with a clinical sensitivity of 100% (95% CI, 82.4-100) and a specificity of 77.3% (95% CI, 64.3-90.3) for LM diagnosis. HER2+ CTCs were detected in the CSF of 40.6% of patients with HER2- BC (median: 500 CTC, range: 13 to 28 320).
CONCLUSIONS: The clinical sensitivity of CTC detection in CSF with the CellSearch® system for LM diagnosis is higher than that of CSF cytology. CTC detection in patients with negative cytology, however, must be further investigated. The finding of HER2+ CTCs in patients with HER2- BC suggests that the HER2 status of LM should be evaluated to increase the treatment opportunities for these patients. |
Publication |
Clinical Chemistry |
Volume |
68 |
Issue |
10 |
Pages |
1311-1322 |
Date |
2022-10-06 |
Journal Abbr |
Clin Chem |
Language |
eng |
DOI |
10.1093/clinchem/hvac127 |
ISSN |
1530-8561 |
Short Title |
Detection of Circulating Tumor Cells in Cerebrospinal Fluid of Patients with Suspected Breast Cancer Leptomeningeal Metastases |
Library Catalog |
PubMed |
Extra |
PMID: 35953885 |
Tags |
Adult, Biomarkers, Tumor, Breast Neoplasms, Cell Count, clinic, Female, Humans, Neoplastic Cells, Circulating, Prospective Studies, Sensitivity and Specificity |
Date Added |
2023/10/16 - 15:35:57 |
Date Modified |
2023/10/16 - 17:41:51 |
Notes and Attachments |
PubMed entry (Attachment) |