| Added by | amaraver |
|---|---|
| Group name | EquipeAM |
| Item Type | Journal Article |
| Title | Coprs inactivation leads to a derepression of LINE1 transposons in spermatocytes |
| Creator | Paul et al. |
| Author | Conception Paul |
| Author | Hélène Delpech |
| Author | Delphine Haouzi |
| Author | Samir Hamamah |
| Author | Claude Sardet |
| Author | Eric Fabbrizio |
| Abstract | Repression of retrotransposons is essential for genome integrity during germ cell development and is tightly controlled through epigenetic mechanisms. In primordial germ cells, protein arginine N-methyltransferase (Prmt5) is involved in retrotransposon repression by methylating Piwi proteins, which is part of the piRNA pathway. Here, we show that in mice, genetic inactivation of coprs (which is highly expressed in testis and encodes a histone-binding protein required for the targeting of Prmt5 activity) affects the maturation of spermatogonia to spermatids. Mass spectrometry analysis revealed the presence of Miwi in testis protein lysates immunoprecipitated with an anti-Coprs antibody. The observed deregulation of Miwi and pachytene pre-piRNAs levels and the derepression of LINE1 repetitive sequences observed in coprs-/- mice suggest that Coprs is implicated in genome surveillance mechanisms. |
| Publication | FEBS open bio |
| Volume | 9 |
| Issue | 1 |
| Pages | 159-168 |
| Date | Jan 2019 |
| Journal Abbr | FEBS Open Bio |
| Language | eng |
| DOI | 10.1002/2211-5463.12562 |
| ISSN | 2211-5463 |
| Library Catalog | PubMed |
| Extra | PMID: 30652083 PMCID: PMC6325579 |
| Tags | coprs, Miwi, original, piRNA, spermatocyte, teratozoospermia, testis |
| Date Added | 2019/05/16 - 11:53:02 |
| Date Modified | 2019/05/16 - 11:53:15 |
| Notes and Attachments | Full Text (Attachment) PubMed entry (Attachment) |
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