| Added by | chplanque |
|---|---|
| Group name | EquipeADV |
| Item Type | Journal Article |
| Title | The PIWI protein Aubergine recruits eIF3 to activate translation in the germ plasm |
| Creator | Ramat et al. |
| Author | Anne Ramat |
| Author | Maria-Rosa Garcia-Silva |
| Author | Camille Jahan |
| Author | Rima Naït-Saïdi |
| Author | Jérémy Dufourt |
| Author | Céline Garret |
| Author | Aymeric Chartier |
| Author | Julie Cremaschi |
| Author | Vipul Patel |
| Author | Mathilde Decourcelle |
| Author | Amandine Bastide |
| Author | François Juge |
| Author | Martine Simonelig |
| Abstract | Cell Res. 2020 May;30(5):421-435. doi: 10.1038/s41422-020-0294-9. Epub 2020 Mar 4. ABSTRACT Piwi-interacting RNAs (piRNAs) and PIWI proteins are essential in germ cells to repress transposons and regulate mRNAs. In Drosophila, piRNAs bound to the PIWI protein Aubergine (Aub) are transferred maternally to the embryo and regulate maternal mRNA stability through two opposite roles. They target mRNAs by incomplete base pairing, leading to their destabilization in the soma and stabilization in the germ plasm. Here, we report a function of Aub in translation. Aub is required for translational activation of nanos mRNA, a key determinant of the germ plasm. Aub physically interacts with the poly(A)-binding protein (PABP) and the translation initiation factor eIF3. Polysome gradient profiling reveals the role of Aub at the initiation step of translation. In the germ plasm, PABP and eIF3d assemble in foci that surround Aub-containing germ granules, and Aub acts with eIF3d to promote nanos translation. These results identify translational activation as a new mode of mRNA regulation by Aub, highlighting the versatility of PIWI proteins in mRNA regulation. PMID:32132673 | PMC:PMC7196074 | DOI:10.1038/s41422-020-0294-9 |
| Publication | Cell research |
| Date | 2020-03-06 00:00:00 |
| Language | en |
| DOI | 10.1038/s41422-020-0294-9 |
| URL | https://pubmed.ncbi.nlm.nih.gov/32132673/?utm_source=Firefox&utm_medium=rss&utm_campaign=pubmed-2&utm_content=16WqI68KDOBEDOCNE1P3RxYQYPTwTutpbvUaDhoUuYPuJd459j&fc=20250120064306&ff=20250120064533&v=2.18.0.post9+e462414 |
| Tags | original |
| Date Added | 2025/01/20 - 14:26:34 |
| Date Modified | 2025/01/20 - 15:05:17 |
| Notes and Attachments | (Attachment) |
Institut de Recherche en Cancérologie de
