| Added by | mollevi |
|---|---|
| Last modified by | standudu |
| Group name | EquipeVC |
| Item Type | Journal Article |
| Title | Research Resource: STR DNA profile and gene expression comparisons of human BG-1 cells and a BG-1/MCF-7 clonal variant |
| Creator | Li et al. |
| Author | Y. Li |
| Author | Y. Arao |
| Author | J. M. Hall |
| Author | S. Burkett |
| Author | L. Liu |
| Author | K. Gerrish |
| Author | V. Cavailles |
| Author | K. S. Korach |
| Abstract | Human ovarian cancer BG-1 cells are a valuable in vitro model that has enabled several laboratories to study the estrogenic responses of ovarian cancers. We recently discovered that there are two different BG-1 cell lines being used for experiments, denoted here as BG-1 FR and BG-1 NIEHS, which exhibit striking morphological differences. The objective of this study was to methodically analyze these two BG-1 variants and compare their characteristics. Short tandem repeat analysis revealed that the DNA profile of BG-1 FR cells was unique, yet the Short tandem repeat pattern of BG-1 NIEHS was identical with that of MCF-7 cells. From a cytogenetic analysis, it became apparent that the BG-1 FR line had the same profile as previously reported, whereas the BG-1 NIEHS and MCF-7 cells share a similar genetic display. A significant number of unique chromosomal translocations were observed between the BG-1 NIEHS and MCF-7 cells, suggesting that acquired genotypic differences resulted in the formation of two lines from a common origin. Although all cell types demonstrated a similar estrogen responsiveness in reporter gene assays, a microarray analysis revealed distinct estrogen-responsive gene expression patterns with surprisingly moderate to low overlap. We conclude that BG-1 FR is the original ovarian cancer cell line, whereas the BG-1 NIEHS is a variant from the MCF-7 cells. These findings provide much needed clarification of the identities and characteristics of key cell line models that are widely used to study estrogen action in female reproductive cancers. |
| Publication | Mol Endocrinol |
| Volume | 28 |
| Pages | 2072-81 |
| Date | Dec 2014 |
| Journal Abbr | Molecular endocrinology |
| DOI | 10.1210/me.2014-1229 |
| ISSN | 1944-9917 (Electronic) 0888-8809 (Linking) |
| Tags | Cell Line, Tumor, DNA/*genetics, Female, Genotype, Humans, original, Ovarian Neoplasms/*genetics, Receptors, Estrogen/genetics, Translocation, Genetic/genetics |
| Date Added | 2018/11/14 - 12:10:52 |
| Date Modified | 2019/06/06 - 22:43:29 |
| Notes and Attachments | (Note) (Note) 25321415 (Attachment) |
Institut de Recherche en Cancérologie de
