| Added by | eric_julien |
|---|---|
| Group name | EquipeEJ |
| Item Type | Journal Article |
| Title | RNAi components are required for nuclear clustering of Polycomb group response elements |
| Creator | Grimaud et al. |
| Author | Charlotte Grimaud |
| Author | Frédéric Bantignies |
| Author | Manika Pal-Bhadra |
| Author | Pallavi Ghana |
| Author | Utpal Bhadra |
| Author | Giacomo Cavalli |
| Abstract | Drosophila Polycomb group (PcG) proteins silence homeotic genes through binding to Polycomb group response elements (PREs). Fab-7 is a PRE-containing regulatory element from the homeotic gene Abdominal-B. When present in multiple copies in the genome, Fab-7 can induce long-distance gene contacts that enhance PcG-dependent silencing. We show here that components of the RNA interference (RNAi) machinery are involved in PcG-mediated silencing at Fab-7 and in the production of small RNAs at transgenic Fab-7 copies. In general, these mutations do not affect the recruitment of PcG components, but they are specifically required for the maintenance of long-range contacts between Fab-7 copies. Dicer-2, PIWI, and Argonaute1, three RNAi components, frequently colocalize with PcG bodies, and their mutation significantly reduces the frequency of PcG-dependent chromosomal associations of endogenous homeotic genes. This suggests a novel role for the RNAi machinery in regulating the nuclear organization of PcG chromatin targets. |
| Publication | Cell |
| Volume | 124 |
| Issue | 5 |
| Pages | 957-971 |
| Date | Mar 10, 2006 |
| Journal Abbr | Cell |
| Language | eng |
| DOI | 10.1016/j.cell.2006.01.036 |
| ISSN | 0092-8674 |
| Library Catalog | PubMed |
| Extra | PMID: 16530043 |
| Tags | Animals, Animals, Genetically Modified, Cell Nucleus, Chromosomes, Drosophila melanogaster, Drosophila Proteins, Gene Expression Regulation, Developmental, Gene Silencing, In Situ Hybridization, Fluorescence, Larva, Mutation, original, Phenotype, Polycomb Repressive Complex 1, Repressor Proteins, Response Elements, RNA Interference, RNA, Small Interfering |
| Date Added | 2018/09/26 - 15:50:03 |
| Date Modified | 2019/05/28 - 13:36:13 |
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