| Added by | lklinares |
|---|---|
| Group name | EquipeLL |
| Item Type | Journal Article |
| Title | Isocitrate dehydrogenase 1 mutations prime the all-trans retinoic acid myeloid differentiation pathway in acute myeloid leukemia |
| Creator | Boutzen et al. |
| Author | Héléna Boutzen |
| Author | Estelle Saland |
| Author | Clément Larrue |
| Author | Fabienne de Toni |
| Author | Lara Gales |
| Author | Florence A. Castelli |
| Author | Mathilde Cathebas |
| Author | Sonia Zaghdoudi |
| Author | Lucille Stuani |
| Author | Tony Kaoma |
| Author | Romain Riscal |
| Author | Guangli Yang |
| Author | Pierre Hirsch |
| Author | Marion David |
| Author | Véronique De Mas-Mansat |
| Author | Eric Delabesse |
| Author | Laurent Vallar |
| Author | François Delhommeau |
| Author | Isabelle Jouanin |
| Author | Ouathek Ouerfelli |
| Author | Laurent Le Cam |
| Author | Laetitia K. Linares |
| Author | Christophe Junot |
| Author | Jean-Charles Portais |
| Author | François Vergez |
| Author | Christian Récher |
| Author | Jean-Emmanuel Sarry |
| Abstract | Acute myeloid leukemia (AML) is characterized by the accumulation of malignant blasts with impaired differentiation programs caused by recurrent mutations, such as the isocitrate dehydrogenase (IDH) mutations found in 15% of AML patients. These mutations result in the production of the oncometabolite (R)-2-hydroxyglutarate (2-HG), leading to a hypermethylation phenotype that dysregulates hematopoietic differentiation. In this study, we identified mutant R132H IDH1-specific gene signatures regulated by key transcription factors, particularly CEBP?, involved in myeloid differentiation and retinoid responsiveness. We show that treatment with all-trans retinoic acid (ATRA) at clinically achievable doses markedly enhanced terminal granulocytic differentiation in AML cell lines, primary patient samples, and a xenograft mouse model carrying mutant IDH1. Moreover, treatment with a cell-permeable form of 2-HG sensitized wild-type IDH1 AML cells to ATRA-induced myeloid differentiation, whereas inhibition of 2-HG production significantly reduced ATRA effects in mutant IDH1 cells. ATRA treatment specifically decreased cell viability and induced apoptosis of mutant IDH1 blasts in vitro. ATRA also reduced tumor burden of mutant IDH1 AML cells xenografted in NOD-Scid-IL2r?(null)mice and markedly increased overall survival, revealing a potent antileukemic effect of ATRA in the presence of IDH1 mutation. This therapeutic strategy holds promise for this AML patient subgroup in future clinical studies. |
| Publication | The Journal of Experimental Medicine |
| Volume | 213 |
| Issue | 4 |
| Pages | 483-497 |
| Date | Apr 04, 2016 |
| Journal Abbr | J Exp Med |
| Language | eng |
| DOI | 10.1084/jem.20150736 |
| ISSN | 1540-9538 |
| Library Catalog | PubMed |
| Extra | PMID: 26951332 PMCID: PMC4821643 |
| Tags | Amino Acid Substitution, Animals, Blast Crisis, CCAAT-Enhancer-Binding Proteins, Cell Differentiation, Cell Survival, Female, Granulocytes, HL-60 Cells, Humans, Isocitrate Dehydrogenase, Leukemia, Myeloid, Acute, Male, Mice, Mice, Nude, Mutation, Missense, Neoplasm Proteins, original, Tretinoin, Xenograft Model Antitumor Assays |
| Date Added | 2024/12/03 - 09:12:56 |
| Date Modified | 2024/12/03 - 09:12:56 |
| Notes and Attachments | PubMed entry (Attachment) Texte intégral (Attachment) |
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