| Added by | alainmange |
|---|---|
| Group name | PlateformePP2I |
| Item Type | Journal Article |
| Title | The organizer of chromatin topology RIF1 ensures cellular resilience to DNA replication stress |
| Creator | Lebdy et al. |
| Author | Rana Lebdy |
| Author | Julie Patouillard |
| Author | Marion Larroque |
| Author | Serge Urbach |
| Author | Raghida Abou Merhi |
| Author | Christian Larroque |
| Author | Cyril Ribeyre |
| Abstract | Eukaryotic genomes are duplicated from thousands of replication origins that fire sequentially forming a defined spatiotemporal pattern of replication clusters. The temporal order of DNA replication is determined by chromatin architecture and, more specifically, by chromatin contacts that are stabilized by RIF1. Here, we show that RIF1 localizes near newly synthesized DNA. In cells exposed to the DNA replication inhibitor aphidicolin, suppression of RIF1 markedly decreased the efficacy of isolation of proteins on nascent DNA, suggesting that the isolation of proteins on nascent DNA procedure is biased by chromatin topology. RIF1 was required to limit the accumulation of DNA lesions induced by aphidicolin treatment and promoted the recruitment of cohesins in the vicinity of nascent DNA. Collectively, the data suggest that the stabilization of chromatin topology by RIF1 limits replication-associated genomic instability. |
| Publication | Life Science Alliance |
| Volume | 6 |
| Issue | 4 |
| Pages | e202101186 |
| Date | 2023-04 |
| Journal Abbr | Life Sci Alliance |
| Language | eng |
| DOI | 10.26508/lsa.202101186 |
| ISSN | 2575-1077 |
| Library Catalog | PubMed |
| Extra | PMID: 36746532 PMCID: PMC9906048 |
| Tags | Aphidicolin, author, Chromatin, DNA, DNA Replication, original, pp2i, Telomere-Binding Proteins |
| Date Added | 2024/07/22 - 11:32:42 |
| Date Modified | 2024/07/22 - 11:35:12 |
| Notes and Attachments | PubMed entry (Attachment) |
Institut de Recherche en Cancérologie de
