| Added by | mollevi |
|---|---|
| Group name | EquipeMY |
| Item Type | Journal Article |
| Title | Antibody targeting of claudin-1 as a potential colorectal cancer therapy |
| Creator | Cherradi et al. |
| Author | S. Cherradi |
| Author | A. Ayrolles-Torro |
| Author | N. Vezzo-Vié |
| Author | N. Gueguinou |
| Author | V. Denis |
| Author | E. Combes |
| Author | M. Busson |
| Author | L. Canterel-Thouennon |
| Author | C. Mollevi |
| Author | M. Pugnière |
| Author | F. Bibeau |
| Author | M. Ychou |
| Author | P. Martineau |
| Author | C. Gongora |
| Author | M. Del Rio |
| Abstract | BACKGROUND: Metastatic colorectal cancer (mCRC) is one of the major causes of cancer-related death. Despite the substantial progress in mCRC management, it remains important to identify new therapeutic options and biological markers for personalized medicine. Here, we investigated the expression of claudin-1 (CLDN1), a major tight junction transmembrane protein, in the different colorectal cancer (CRC) molecular subtypes and then assessed the anti-tumor effect of a new anti-CLDN1 monoclonal antibody (mAb). METHODS: Gene expression profiling and immunochemistry analysis of normal and tumor tissue samples from patients with stage IV CRC were used to determine CLDN1 gene expression. Then, the 6F6 mAb against CLDN1 extracellular part was generated. Its effect on CRC cell cycle, proliferation, survival and migration was assessed in vitro, using a 3D cell culture system, flow cytometry, clonogenic and migration assays. In vivo, 6 F6 mAb efficacy was evaluated in nude mice after subcutaneous xenografts or intrasplenic injection of CRC cells. RESULTS: Compared with normal mucosa where it was almost exclusively cytoplasmic, in CRC samples CLDN1 was overexpressed (p? |
| Publication | Journal of experimental & clinical cancer research: CR |
| Volume | 36 |
| Issue | 1 |
| Pages | 89 |
| Date | 06 28, 2017 |
| Journal Abbr | J. Exp. Clin. Cancer Res. |
| Language | eng |
| DOI | 10.1186/s13046-017-0558-5 |
| ISSN | 1756-9966 |
| Library Catalog | PubMed |
| Extra | PMID: 28659146 PMCID: PMC5490170 |
| Tags | Animals, Antibodies, Monoclonal, Antineoplastic Agents, Immunological, Biomarkers, Tumor, Cell Cycle, Cell Line, Tumor, Cell Proliferation, Cell Survival, Claudin-1, Colorectal Neoplasms, Disease Models, Animal, Female, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Humans, Intestinal Mucosa, Metastatic, Mice, Molecular Targeted Therapy, Neoplasm Metastasis, Neoplasm Staging, original, Prognosis, Retrospective Studies, Single Photon Emission Computed Tomography Computed Tomography, Xenograft Model Antitumor Assays |
| Date Added | 2018/11/13 - 17:25:57 |
| Date Modified | 2019/05/21 - 13:45:16 |
Institut de Recherche en Cancérologie de
