| Added by | ircm doc |
|---|---|
| Group name | EquipeCTCS |
| Item Type | Journal Article |
| Title | First evidence of AXL expression on circulating tumor cells in metastatic breast cancer patients: A proof-of-concept study |
| Creator | Bardol et al. |
| Author | Thomas Bardol |
| Author | Zahra Eslami-S |
| Author | Doryan Masmoudi |
| Author | Marie Alexandre |
| Author | Marie Duboys de Labarre |
| Author | Véronique D'Hondt |
| Author | Séverine Guiu |
| Author | Keerthi Kurma |
| Author | Laure Cayrefourcq |
| Author | William Jacot |
| Author | Catherine Alix-Panabières |
| Abstract | BACKGROUND: For several years, the AXL tyrosine kinase receptor, a member of the Tyro3-Axl-Mer (TAM) family, has been considered a new strategic target in oncology. AXL overexpression is common in solid tumors and is associated with poor prognosis. In this context, the detection of a subset of circulating tumor cells (CTCs) that express AXL (AXL+ CTCs) could be clinically relevant. METHODS: Immunostaining was performed to assess AXL expression in human breast cancer cell lines. The optimal conditions were established using flow cytometry. Spiking experiments were carried out to optimize the parameters of the CellSearch® system detection test. CTC enumeration and AXL expression were evaluated in patients with metastatic breast cancer (mBC) before treatment initiation. RESULTS: An innovative AXL+ CTC detection assay to be used with the CellSearch® system was developed. In a prospective longitudinal clinical trial, blood samples from 60 patients with untreated mBC were analyzed to detect AXL+ CTCs with this new assay. CTCs were detected in 35/60 patients (58.3%) and AXL+ CTCs were identified in 7 of these 35 patients (11.7% of all patients). CONCLUSION: This newly established AXL+ CTC assay is a promising tool that can be used for liquid biopsy in future clinical trials to stratify and monitor patients with cancer receiving anti-AXL therapies. |
| Publication | Cancer Medicine |
| Volume | 13 |
| Issue | 1 |
| Pages | e6843 |
| Date | 2024-01 |
| Journal Abbr | Cancer Med |
| Language | eng |
| DOI | 10.1002/cam4.6843 |
| ISSN | 2045-7634 |
| Short Title | First evidence of AXL expression on circulating tumor cells in metastatic breast cancer patients |
| Library Catalog | PubMed |
| Extra | PMID: 38132919 PMCID: PMC10807582 |
| Tags | Adult, Aged, AXL, Axl Receptor Tyrosine Kinase, Biomarkers, Tumor, Breast Neoplasms, Cell Line, Tumor, circulating biomarker, Female, Humans, liquid biopsy, metastatic breast cancer, Middle Aged, Neoplasm Metastasis, Neoplastic Cells, Circulating, original, Proof of Concept Study, Prospective Studies, Proto-Oncogene Proteins, Receptor Protein-Tyrosine Kinases |
| Date Added | 2025/01/13 - 11:59:45 |
| Date Modified | 2025/01/16 - 09:48:25 |
| Notes and Attachments | PubMed entry (Attachment) Texte intégral (Attachment) |
Institut de Recherche en Cancérologie de
